Effect of sodium alginate-based hydrogel loaded with lutein on gut microbiota and inflammatory response in DSS-induced colitis mice  

作　　者：Pengxiang Xu Shuwei Luo Jiangfeng Song Zhuqing Dai Dajing Li Cai’e Wu 

机构地区：[1]Institute of Agro-product Processing,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China [2]College of Light Industry and Food Engineering,Nanjing Forestry University,Nanjing 210037,China

出　　处：《Food Science and Human Wellness》2023年第6期2428-2439,共12页食品科学与人类健康（英文）

基　　金：supported by the Independent Innovation Fund Project of Agricultural Science and Technology in Jiangsu Province(Project No.CX(20)3047).

摘　　要：In order to effectively deliver lutein to the inflamed colon and better exert its pharmacological activity,this paper constructed a sodium alginate hydrogel-based delivery system loaded with lutein nanoparticles,evaluated the regulation on the expression and secretion of related inflammatory factors in mice with colitis,and its impact on intestinal microbial environment.The results showed that comparing lutein crystal and its nanoparticle,lutein hydrogel alleviated dextran sodium sulfate(DSS)-induced colitis in mice more effectively by adjusting fecal heme content,colon tissue damage,and inflammatory factor levels.Moreover,lutein hydrogel increased the expression of intestinal tight junction proteins zonula occluden-1(ZO-1),claudin-1 and occludin to maintain the integrity of the intestinal-barrier,inhibited the nuclear factor-κB(NF-κB)pathway and reduced expression and secretion of inflammatory factors including tumour necrosis factor-α(TNF-α),inducible nitric oxide synthase(iNOS),NOD-like receptors 3(NLRP3)and interleukin(IL)-1β.In addition,the intestinal microbial environment of mice with colitis was improved by down-regulating the relative abundance of Desulfovibrionaceae and up-regulating the relative abundance of Erysipelotrichaceae and Rikenellaceae.As a slow-release carrier to load lutein nanoparticles,sodium alginate-based hydrogel has potential application prospect.

关 键 词：LUTEIN HYDROGEL COLITIS Intestinal microbiota Inflammation regulation 

分 类 号：TQ460.4[化学工程—制药化工]