长链非编码RNA LINC02560在肝细胞肝癌中的表达及其临床意义  

Expression of LINC02560 in hepatocellular carcinoma tissues and cells and its clinical significance

作　　者：龙朴泽 刘肸琳 罗春英[2,3] LONG Pu-ze;LIU Xi-lin;LUO Chun-ying(Graduate School,Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China;Department of Pathology,Affiliated Hospital of Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China;The Key Laboratory of Molecular Pathology(For Hepatobiliary Diseases)of Guangxi,Baise 533000,Guangxi,China)

机构地区：[1]右江民族医学院研究生学院,广西百色533000 [2]右江民族医学院附属医院病理科,广西百色533000 [3]右江民族医学院广西肝胆疾病分子病理重点实验室,广西百色533000

出　　处：《川北医学院学报》2023年第4期435-439,共5页Journal of North Sichuan Medical College

基　　金：广西壮族自治区自然科学基金项目(2020GXNSFAA259052);广西壮族自治区科技计划创新研究团队项目(2017GXNSFG);百色市区域多发病联合专项计划课题(20224104)。

摘　　要：目的:探讨长链非编码RNA(LncRNA)LINC02560在肝细胞肝癌(HCC)中的表达及其临床意义。方法:收集40例手术切除的HCC组织及配对的癌旁组织,采用RT-qPCR检测LINC02560在HCC组织和癌旁组织中的表达,分析LINC02560表达与患者不同临床病理特征的相关性及其对肝癌的诊断价值。结果:LINC02560在HCC肿瘤组织中表达水平高于癌旁肝组织(P<0.05);LINC02560表达高低与HCC患者肿瘤大小、MVI(肝癌的微血管侵犯)分期存在相关性(P<0.05)。肿瘤直径大于5 cm的肿瘤组织LINC02560倾向于高表达;M1~M2患者LINC02560表达水平高于M0患者肿瘤组织;LINC02560高表达组的Ki-67阳性表达水平高于低表达组(P<0.05)。ROC曲线分析显示,LINC02560诊断肝癌的ROC曲线下面积(AUC)为0.8169(95%CI:0.726~0.908)。结论:LINC02560在HCC肿瘤组织和细胞中高表达,其表达高低与肿瘤大小、微血管侵犯相关,可能作为促癌基因促进HCC肿瘤细胞增殖和侵袭能力的生物学功能,有望成为HCC早期分子诊断指标和分子治疗靶点。Objective:To investigate the expression of long non-coding RNA(lncRNA)LINC02560 in hepatocellular carcinoma(HCC)and its clinical significance.Methods:40 cases of surgically resected HCC tissues and paired adjacent tissues were selected.RT-qPCR was used to detect the expression difference of LINC02560 between HCC tissues and adjacent tissues.The correlation between LINC02560 expression and clinicopathological features,and its diagnostic value for liver cancer were analyzed.Results:The expression level of LINC02560 in HCC tumor tissues was higher than that in adjacent liver tissues(P<0.05).LINC02560 expression was correlated with tumor size and MVI(microvascular invasion of liver cancer)stage in HCC patients(P<0.05).LINC02560 expression tended to be high in tumor tissues with tumor diameter greater than 5 cm,and LINC02560 expression in M1~M2 patients was higher than that in M0 patients.The positive expression of Ki-67 in LINC02560 high expression group was higher than that in LINC02560 low expression group(P<0.05).ROC curve analysis showed that the area under the ROC curve of LINC02560 in the diagnosis of liver cancer was 0.8169(95%CI:0.726~0.908).Conclusion:LINC02560 is highly expressed in HCC tumor tissues and cells,and its expression level is correlated with tumor size and microvascular invasion,suggesting that LINC02560 may play a biological function as a oncogenic gene to promote the proliferation and invasion of HCC tumor cells,which is expected to be an early molecular diagnostic index and molecular therapeutic target for HCC.

关 键 词：肝细胞肝癌 长链非编码RNA 02560 增殖 临床病理特征 

分 类 号：R735.7[医药卫生—肿瘤]