基于MSR1 mRNA和蛋白在泛癌组织中表达的生物信息学分析及其意义  

Bioinformatics analysis based on expressions of MSR1 mRNA and protein in pan-cancer tissue and its significance

作　　者：张德洪 郑明珠 李家秋 路中[2] ZHANG Dehong;ZHENG Mingzhu;LI Jiaqiu;LU Zhong(School of Clinical Medicine,Weifang Medical University,Weifang 261053,China;Department of Oncology,Affiliated Hospital of Weifang Medical University,Weifang 261031,China)

机构地区：[1]潍坊医学院临床医学院,山东潍坊261053 [2]潍坊医学院附属医院肿瘤科,山东潍坊261031

出　　处：《吉林大学学报（医学版）》2023年第2期425-439,共15页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金项目(81902404);山东省自然科学基金资助项目(ZR2019BH009);山东省卫健委医药卫生科技发展计划项目(2017WS403)。

摘　　要：目的:通过生物信息学分析探讨巨噬细胞清道夫受体1 (MSR1)在泛癌组织中的表达水平、患者生存情况和免疫特性,阐明MSR1作为新的生物标志物对肿瘤的诊断、预后和免疫治疗的价值。方法:采用临床生信之家数据库和Sangerbox数据库分析正常组织和肿瘤组织中MSR1 mRNA表达水平,采用人类蛋白质图谱(HPA)数据库分析MSR1蛋白表达情况,采用单因素生存分析和Kaplan-Meier分析评估MSR1对预后的价值,采用肿瘤免疫单细胞中心(TISCH)数据库的单细胞测序结果分析MSR1在各种细胞类型中的表达情况,采用肿瘤免疫估计资源(TIMER2.0)、肿瘤免疫共基因小鼠(TISMO)、肿瘤免疫功能障碍与排斥(TIDE)和基因集癌症分析(GSCA)数据库分析泛癌组织中MSR1表达水平与免疫细胞浸润、免疫检查点基因表达和免疫治疗反应之间的相关性。结果:与正常组织比较,在17种肿瘤组织中MSR1 mRNA表达水平上调,包括乳腺浸润性癌(BRCA)、结肠腺癌(COAD)、食道癌(ESCA)、多形性胶质母细胞瘤(GBM)、头颈部鳞状细胞癌(HNSC)、肾透明细胞癌(KIRC)、肾性肾乳头状细胞癌(KIRP)、脑低级别胶质瘤(LGG)、肝细胞癌(LIHC)、卵巢浆液性囊腺癌(OV)、胰腺腺癌(PAAD)、前列腺癌(PRAD)、皮肤黑色素瘤(SKCM)、胃腺癌(STAD)、睾丸生殖细胞肿瘤(TGCT)、甲状腺癌(THCA)和子宫颈肉瘤(UCS)(P<0.01);与正常组织比较,乳腺癌、子宫内膜癌、肝癌、卵巢癌、皮肤黑色素瘤、睾丸癌、胰腺癌和前列腺癌组织中MSR1蛋白表达水平也有不同程度的升高。在膀胱尿路上皮癌(BLCA)[风险比(HR)=1.01, P=0.047, 95%CI (1.00, 1.03)]、 LGG [HR=1.03, P<0.001,95%CI (1.02, 1.04)]、 LIHC [HR=1.04, P=0.007, 95%CI (1.01, 1.07)]、 OV [HR=1.02, P=0.028, 95%CI (1.00, 1.03)]、 STAD [HR=1.02, P=0.016, 95%CI (1.00,1.04)]、 THCA [HR=1.06, P=0.006, 95%CI (1.02, 1.11)]和葡萄膜黑色素瘤(UVM)[HR=1.18,P=0.044,95%CI (1.00,1.40)]中MSR1高表达与患者较差的总Objective:To discuss the expression level of macrophage scavenger receptor 1(MSR1)in pan-cancer tissue,survival and immune characteristics of the patients by bioinformatics analysis,and to elucidate the value of MSR1 as a new biomarker for the tumor diagnosis,prognosis,and immunotherapy.Methods:Clinical Bioinformatic Database and Sangerbox Database were used to analyze the expression levels of MSR1 mRNA in normal tissue and tumor tissue,the expression of MSR1 protein was analyzed by The Human Protein Atlas(HPA)Database,and univariate survival analysis and Kaplan-Meier analysis were used to evaluate the prognostic value of MSR1,and the expressions of MSR1 in various types of cells were analyzed with single-cell sequencing results from Tumor Immune Single-cell Hub(TISCH)Database;Tumor Immune Estimation Resource(TIMER2.0),Tumor Immune Syngeneic Mouse(TISMO),Tumor Immune Dysfunction and Exclusion(TIDE),and Gene Set Cancer Analysis(GSCA)Databases were used to analyze the correlations between the MSR1 expression level in pan-cancer tissue and immune cell infiltration,immune checkpoint gene expression,and immune treatment response.Results:Compared with normal tissue,the expression levels of MSR1 mRNA in 17 kinds of tumor tissues including breast invasive carcinoma(BRCA),colon adenocarcinoma(COAD),esophageal carcinoma(ESCA),glioblastoma multiforme(GBM),head and neck squamous cell carcinoma(HNSC),kidney renal clear cell carcinoma(KIRC),kidney renal papillary cell carcinoma(KIRP),brain low-grade glioma(LGG),liver hepatocellular carcinoma(LIHC),ovarian serous cystadenocarcinoma(OV),pancreatic adenocarcinoma(PAAD),prostate adenocarcinoma(PRAD),skin cutaneous melanoma(SKCM),stomach adenocarcinoma(STAD),testicular germ cell tumor(TGCT),thyroid carcinoma(THCA),and uterine carcinosarcoma(UCS)were increased(P<0.01);compared with normal tissue,the expression levels of MSR1 protein in breast cancer,endometrial cancer,liver cancer,ovarian cancer,skin melanoma,testis cancer,pancreatic cancer,and prostate cancer tissues were increased

关 键 词：巨噬细胞清道夫受体1 泛癌 免疫治疗 肿瘤微环境 脂质代谢 

分 类 号：R730.3[医药卫生—肿瘤]