自主运动训练通过调节Caspase-3的活性抑制人BRCA1突变乳腺癌的增殖与生长  

Voluntary Exercise Training Attenuated the Proliferation and Growth of Human Breast Cancer with BRCA1 Mutation by Regulating Caspase-3 Activity

作　　者：胡滔 吴怡[2] 耿文达 章意坚 贺瑄 李珊珊 习杨彦彬 邓丽玲 HU Tao;WU Yi;GENG Wenda;ZHANG Yijian;HE Xuan;LI Shanshan;XIYANG Yanbin;DENG Liling(Dept.of Laboratory Medicine,The 3rd People’s Hospital of Yunnan Province,Kunming Yunnan 650200;Science and Technology Achievement Incubation Center,Kunming Medical University,Kunming Yunnan 650500;School of Basic Medicine Kunming Medical University,Kunming Medical University,Kunming Yunnan 650500;Dept.of Gynecology,Hunan Provincial TCM Hospital,Zhuzhou Hunan 412008,China)

机构地区：[1]云南省第三人民医院医学检验科,云南昆明650200 [2]昆明医科大学科技成果孵化中心,云南昆明650500 [3]昆明医科大学基础医学院,云南昆明650500 [4]湖南省直中医医院妇科,湖南株洲412008

出　　处：《昆明医科大学学报》2023年第4期31-39,共9页Journal of Kunming Medical University

基　　金：云南省教育厅科学研究基金资助项目(2023Y0608)。

摘　　要：目的探讨自主运动训练在乳腺癌发生发展中的作用及其机制。方法将携带原发性人乳腺癌基因1(breast cancer gene 1,BRCA-1)突变基因的癌细胞在体外进行培养,用连续14 d自主跑轮运动鼠血清以及静坐鼠血清处理BRCA-1突变肿瘤细胞,并将上述条件血清培养的乳腺癌细胞注射到BALB/c裸鼠皮下,观察经过不同处理后体内外BRCA-1突变乳腺癌细胞及移植瘤的情况。结果在体外,与静坐鼠血清处理组相比,自主运动鼠血清处理后抑制了BRCA-1突变乳腺癌细胞的活力、生长,促进其凋亡,差异具有统计学意义(P<0.05);在体内,自主运动鼠血清培养的BRCA-1突变乳腺癌细胞移植瘤较静坐对照组体积减小、生长缓慢(P<0.05),体内外自主运动组均呈现出增强的Caspase-3活性(P<0.05)。结论自主跑轮运动可能通过增强Caspase3的活性、抑制了BRCA1突变乳腺癌细胞以及其在BALB/c裸鼠皮下异种移植瘤的增殖、促进肿瘤细胞凋亡,从而抑制其体内外的生长。Objective This study aims to explore the role and mechanism of voluntary exercise training in the development and progression of breast cancer.Methods Primary human Breast Cancer gene 1-mutation(BRCA-1 mutation)cancer cells were cultured in vitro,before treated with the serum of wheel running rats and sedentary rats for 14 consecutive days.The cells cultured in above conditions were injected subcutaneously into BALB/c nude mice to observe the in vitro and in vivo breast cancer cells with BRCA-1 mutation and tumor transplantation after different interventions.Results In vitro,compared with sedentary rat serum group,the activity and growth of BRCA-1 mutant breast cancer cells were inhibited and apoptosis was promoted after in the wheel running group.In vivo,the grafts of BRCA-1 mutated breast cancer cells cultured from in the wheel running group decreased in volume and grew slower compared with the sedentary group,and the wheel running group showed enhanced Caspase-3 activity both in vivo and in vitro.Conclusion Voluntary wheel running may attenuate the growth of BRCA1 mutant breast cancer cells and their proliferation and apoptosis in BALB/c nude mice by enhancing the activity of Caspase3,thus inhibiting the growth of breast cancer cells in vitro and in vivo.

关 键 词：乳腺癌 乳腺癌基因1 自主跑轮训练 增殖 凋亡 

分 类 号：R739.9[医药卫生—肿瘤]