针刺对形觉剥夺性近视豚鼠巩膜形态及组织中HIF-1α、SOD、MDA水平的影响  

Effects of acupuncture on the scleral morphology and levels of hypoxia-inducible factor-1α,superoxide dismutase and malondialdehyde in the scleral tissues of form-deprivation myopic guinea pigs

作　　者：邓柔 张曦文 杨轲 路雪婧 DENG Rou;ZHANG Xiwen;YANG Ke;LU Xuejing(Chengdu University of Traditional Chinese Medicine,Chengdu 610072,Sichuan Province,China;Eye School of Chengdu University of Traditional Chinese Medicine,Key Laboratory of Sichuan Province Ophthalmopathy Prevention&Cure and Visual Function Protection with Traditional Chinese Medicine Laboratory,Chengdu 610072,Sichuan Province,China)

机构地区：[1]成都中医药大学,四川省成都市610072 [2]成都中医药大学眼科学院,中医药眼病防治与视功能保护四川省重点实验室,四川省成都市610072

出　　处：《眼科新进展》2023年第4期260-265,共6页Recent Advances in Ophthalmology

基　　金：国家自然科学基金项目(编号:82174444);“杏林学者”学科人才科研提升计划项目(编号:YXRC2020003)。

摘　　要：目的 探究针刺对形觉剥夺性近视(FDM)豚鼠巩膜组织形态特点及巩膜组织中缺氧诱导因子1α(HIF-1α)、超氧化物歧化酶(SOD)、丙二醛(MDA)水平的影响。方法 选取普通级3周龄健康三色豚鼠72只,随机分为空白组、模型组和针刺组(各24只)。用半透明气球作为眼罩分别固定于模型组和针刺组豚鼠右眼及头面部建立FDM模型,左眼充分暴露作自身对照;针刺组在豚鼠模型建立当天起,于每日相同时间对豚鼠进行针刺和电针干预治疗,空白组豚鼠不做任何干预。记录每组豚鼠造模前、造模后2周及4周的眼轴长度和屈光度。造模后4周处死豚鼠,光学显微镜下观察巩膜形态变化,Western blot检测各组豚鼠巩膜中HIF-1α蛋白表达,比色法测定各组豚鼠巩膜中SOD活力和MDA含量。结果 造模后2周和4周,与空白组比较,模型组及针刺组豚鼠遮盖眼近视屈光度及眼轴长度均明显增加(均为P<0.05);与模型组比较,针刺组豚鼠遮盖眼近视屈光度减小,眼轴增长明显减缓(均为P<0.05)。造模后4周,与空白组比较,模型组豚鼠巩膜厚度明显变薄,胶原纤维分布稀疏,有明显纤维空隙且排列不规则;与模型组相比,针刺组豚鼠巩膜层增厚,与胶原纤维分离程度较小且排列更加规则。造模后4周,与空白组相比,模型组豚鼠巩膜中HIF-1α蛋白表达显著升高(P<0.01);与模型组相比,针刺组豚鼠巩膜中HIF-1α蛋白表达显著降低(P<0.01)。造模后4周,与空白组相比,模型组豚鼠巩膜中SOD活性显著降低,MDA含量显著提高(均为P<0.01);与模型组相比,针刺组豚鼠巩膜中SOD活性显著升高,MDA含量显著降低(均为P<0.05)。结论 针刺治疗可通过调节FDM豚鼠巩膜中HIF-1α、SOD及MDA的表达,改善巩膜缺氧及抗氧化应激状态,延缓近视的发生发展。Objective To investigate the effects of acupuncture on scleral morphological characteristics and the levels of hypoxia-inducible factor-1α(HIF-1α),superoxide dismutase(SOD)and malondialdehyde(MDA)in the scleral tissues of guinea pigs with form-deprivation myopia(FDM).Methods Seventy-two conventional healthy three-week-old Britain guinea pigs were included and randomly divided into the blank group,model group,and acupuncture group(24 in each group).Translucent balloons were used as eye masks fixed on the right eye,head,and face of guinea pigs in the model group and the acupuncture group to establish the FDM model,while the left eye was fully exposed as the self-control.The guinea pigs in the acupuncture group were treated with acupuncture and electro-acupuncture at the same time every day since the establishment of the model,while guinea pigs in the blank group did not receive any intervention.The axial length and diopter of guinea pigs in each group were recorded before,2 weeks and 4 weeks after modeling.The guinea pigs were sacrificed after 4 weeks of modeling.The change in scleral morphological characteristics were observed by light microscopy.The protein expressions of HIF-1αin the sclera of guinea pigs were detected by Western blot.The activity of SOD and content of MDA were determined by the colorimetric method.Results At 2 weeks and 4 weeks after modeling,compared with the blank group,the myopia diopter and axial length of the covered eyes of guinea pigs in the model group and the acupuncture group significantly increased(all P<0.05);compared with the model group,the myopia diopter of the covered eyes decreased and axial length of the covered eyes showed a significant slowdown in the increase in the acupuncture group(all P<0.05).At 4 weeks after modeling,compared with the blank group,the sclera layer of guinea pigs in the model group significantly thinned,with sparse collagen fibers,obvious spaces between fibers,and irregular arrangement of fibers;compared with the model group,the scleral layer of guinea

关 键 词：形觉剥夺性近视 豚鼠 针刺 巩膜 缺氧诱导因子1Α 超氧化物歧化酶 丙二醛 

分 类 号：R778[医药卫生—眼科]