SD大鼠骨髓基质干细胞的全骨髓培养与鉴定  

Whole bone marrow culture and identification of bone marrow stromal stem cells in SD rats

作　　者：邓娟 左右清 王念[2] 胡熙苒 曾礼 DENG Juan;ZUO Youqing;WANG Nian;HU Xiran;ZENG Li(Clinical College,Xiangtan Medical and Health Vocational Fechnical College,Human 411104;School of Basic Medicine,Xiangya School of Medicine,Central South Umiversity)

机构地区：[1]湘潭医卫职业技术学院临床学院,湖南411104 [2]中南大学湘雅医学院基础医学院

出　　处：《交通医学》2023年第1期6-8,13,共4页Medical Journal of Communications

基　　金：湘潭医卫职业技术学院2021年度重点自科项目(ZRKX2021-04)。

摘　　要：目的:采用体外全骨髓培养法培养SD大鼠骨髓基质干细胞(BMSCs)并进行鉴定。方法:采用全骨髓贴壁法分离扩增大鼠骨髓基质干细胞,采用流式细胞仪检测细胞表面分子CD29、CD34、CD45的表达。结果:全骨髓培养法能迅速、有效地培养和获得大量骨髓基质干细胞,流式细胞仪检测显示CD29阳性率为99.0%,CD34阳性率1.5%,CD45阳性率0.8%。结论:全骨髓贴壁培养法是获得骨髓基质干细简捷、高效、经济的途径,可以获得较纯的BMSCs。Objective:Bone marrow stromal stem cells(BMSCs)of SD rats were cultured and identified by the method of whole bone marrow culture in vitro.Methods:Mouse bone marrow stromal stem cells were isolated and enlarged by the whole bone marrow adherent method,and the expressions of CD29,CD34 and CD45 on the cell surface were detected by flow cytometry.Results:The whole bone marrow culture method can rapidly and effectively culture and obtain a large number of bone marrow stromal stem cells.The positive rate of CD29,CD34 and CD45 were 99.0%,1.5%and 0.8%respectively.Conclusion:The whole bone marrow adherent culture is a simple,efficient and economical way to obtain bone marrow stromal stem cells,and can obtain relatively pure BMSCs.

关 键 词：骨髓基质干细胞 全骨髓培养法 阳性率 

分 类 号：R394.2[医药卫生—医学遗传学]