陈皮挥发油GC-MS分析与橙皮苷含量测定  

GC-MS Analysis of Volatile Oil in Tangerine Peel and Determination of Hesperidin Content

作　　者：徐健 王晓东 陈亮 刘卉 黄晓德 王琦 王思源 李海涛[1] 陈斌 曹鹏[1,4] Xu Jian;Wang Xiaodong;Chen Liang;Liu Hui;Huang Xiaode;Wang Qi;Wang Siyuan;Li Haitao;Chen Bin;Cao Peng(Nanjing University of Chinese Medicine,Nanjing 210023,China;Nanjing Institute for Comprehensive Utilization of Wild Plants,Nanjing 211111,China;Hangzhou Yunzhongyu Network Technology Co.,Ltd.,Hangzhou 310000,China;Affiliated Hospital of Integrated Traditional Chinese and Western Medicine,Nanjing University of Chinese Medicine,Nanjing 210028,China;Dandong Agricultural and Rural Development Service Center,Dandong 118000,China)

机构地区：[1]南京中医药大学,江苏南京210023 [2]南京野生植物综合利用研究所,江苏南京211111 [3]杭州云中羽网络科技有限公司,浙江杭州310000 [4]南京中医药大学附属中西医结合医院,江苏省中医药研究院,江苏南京210028 [5]丹东市农业农村发展服务中心,辽宁丹东118000

出　　处：《中国野生植物资源》2023年第4期39-42,共4页Chinese Wild Plant Resources

基　　金：供销合作社归口标准体系与行业品牌建设项目(2021GH-ZD-05)。

摘　　要：目的:分析陈皮挥发油中化学成分并测定橙皮苷的含量。方法:以陈皮为主要研究实验对象,采用水蒸汽蒸馏法和溶剂热回流提取法分别提取陈皮挥发油及提取物,用气相色谱-质谱联用(GC-MS)方法和高效液相色谱(HPLC)法分别对其主要的化学成分以及橙皮苷的含量进行鉴定和分析。结果:通过分析得出陈皮挥发油中D-柠檬烯成分含量相对较高,占总量的94.15%,此外含量较高的还有γ-萜品烯和β-月桂烯,其相对含量为3.27%和1.20%。而橙皮苷在12.5~200μg/mL范围内的峰面积与进样浓度线性关系良好(R2=0.998),平均加样回收率为99.32%,其相对标准偏差(RSD)为2.47%。结论:采用高效液相色谱法测定陈皮中橙皮苷的含量,重现性和稳定性较好,操作简便,可作为快速、灵敏、准确检测橙皮苷的一种方法。Objective:To analyze the chemical constituents of volatile oil and determine the content of hesperidin in Citrus reticulata pericarpium.Methods:Tangerine peel was the main research object.Volatile oil and extracts were extracted by steam distillation and solvent thermal reflux extraction,respectively.The chemical composition of volatile oil were determined by gas chromatography‑mass spectrometry(GC-MS)method,and the content of hesperidin were identified by high performance liquid chromatography(HPLC)method.Results:The content of D-limonene was relatively high in the volatile oil of tangerine peel,accounting for 94.15%of the total.In addition,the higher content ofγ-terpinene andβ-myrcene had the relative content of 3.27%and 1.20%.The peak area of hesperidin ranging from 12.5 to 200μg/ml was linearly related to the injection concentration(R2=0.998).The average sample recovery rate was 99.32%,and the relative standard deviation(RSD)was 2.47%.Conclusion:The content of hesperidin in tangerine peel was determined by HPLC,with good reproducibility,stability,and easy operation.It could be used as a method for rapid,sensitive and accurate detection of hesperidin.

关 键 词：陈皮 挥发油 橙皮苷 气质联用 高效液相色谱 

分 类 号：R917[医药卫生—药物分析学]