不同海拔黄果柑果实有机酸代谢的生态响应  

Ecological response of organic acid metabolism of Huangguogan fruits at different altitudes

作　　者：吴娇 龚成宇 陈超群 陈红旭 刘俊宏 唐文静 初元琦 杨文龙 张瑶 龚荣高[1] WU Jiao;GONG Chengyu;CHEN Chaoqun;CHEN Hongxu;LIU Junhong;TANG Wenjing;CHU Yuanqi;YANG Wenlong;ZHANG Yao;GONG Ronggao(College of Horticulture,Sichuan Agricultural University,Chengdu 611130,China)

机构地区：[1]四川农业大学园艺学院,四川成都611130

出　　处：《浙江农业学报》2023年第4期853-861,共9页Acta Agriculturae Zhejiangensis

基　　金：国家重点研发计划项目(2017YFC0505104)。

摘　　要：为探究大渡河干暖河谷区黄果柑果实有机酸积累与不同海拔高度的关联性,在同一山体选取了海拔为875 m、1014 m和1174 m的7年生黄果柑,研究有机酸代谢相关指标在果实发育过程中的变化规律。结果表明:黄果柑果实有机酸主要是柠檬酸,占比75%以上,其次是苹果酸和奎宁酸;柠檬酸在后期大量降解,并且海拔越高,柠檬酸含量越低;随着海拔升高果实柠檬酸降解阶段细胞质顺乌头酸酶(ACO)活性和异柠檬酸脱氢酶(NADP-IDH)活性显著提升,同时海拔的升高提前启动ACO基因表达和增加NADP-IDH基因表达量。不同海拔间果实有机酸含量差异取决于果实发育中后期柠檬酸的降解量;引起不同海拔间有机酸含量差异的主要酶是细胞质ACO、NADP-IDH和磷酸烯醇式丙酮酸羧化酶(PEPC);海拔的升高可能会通过提前启动ACO基因表达和增加NADP-IDH基因表达量来提升细胞质ACO及NADP-IDH活性,以促进果实柠檬酸的降解。To reveal the relationship between Huangguogan fruit organic acid accumulation and different altitudes in the dry and warm valley of Dadu River,seven-year-old Huangguogan with altitudes of 875 m,1014 m and 1174 m were selected on the same mountain to study the changes of organic acid metabolism related indicators during fruit development.The results showed that the fruit organic acid was mainly citrate acid,accounting for more than 75%,followed by malic acid and quinic acid;the citric acid content was largely degraded in the later stage,and the higher the altitude,the lower the citric acid content;as the altitude increased significantly enhanced the cytoplasmic ACO activity and NADP-IDH activity in the citric acid degradation stage of the fruit.At the same time,the increase of altitude started ACO gene expression and increased NADP-IDH gene expression in advance.The difference in fruit organic acid content between different altitudes depended on the degradation of citric acid in the middle and late stages of fruit development;the main enzymes that caused the difference in organic acid content between different altitudes were cytoplasmic ACO,NADP-IDH and PEPC.Increased altitude might increase the activity of cytoplasmic ACO and NADP-IDH by activating ACO gene expression in advance and increasing NADP-IDH gene expression to promote the degradation of citric acid in fruit.

关 键 词：海拔 黄果柑 果实发育 有机酸代谢 响应机制 基因表达 

分 类 号：S666.1[农业科学—果树学]