霞多丽葡萄果实降异戊二烯香气物质积累及代谢酶活变化分析  

Analysis for accumulation of norisoprenoids and changes of metabolic enzyme activities of Chardonnay grape berries

作　　者：张祯 崔媛媛 陈春霞 冯丽丹 赵勇 李霁昕 把灵珍 孔祥锦 张煜 蒋玉梅 ZHANG Zhen;CUI Yuanyuan;CHEN Chunxia;FENG Lidan;ZHAO Yong;LI Jixin;BA Lingzhen;KONG Xiangjin;ZHANG Yu;JIANG Yumei(Gansu Key Laboratory of Viticulture and Enology,Research and Development Center of Wine Industry in Gansu Province,College of Food Science and Engineering,Gansu Agricultural University,Lanzhou 730070,China;Ecological Agricultural Demonstration Plantation Area of Gansu Mogao Industrial Development Limited Company,Wuwei 733006,Gansu,China)

机构地区：[1]甘肃农业大学食品科学与工程学院,甘肃省葡萄与葡萄酒工程学重点实验室,甘肃省葡萄与葡萄酒产业技术中心,甘肃兰州730070 [2]甘肃莫高实业发展股份有限公司生态农业示范种植园区,甘肃武威733006

出　　处：《浙江农业学报》2023年第4期931-941,共11页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金地区项目(32060514)。

摘　　要：为确定霞多丽葡萄果实降异戊二烯香气物质的生成和积累规律,试验于2020和2021年连续两个生产季,在果实幼果(花后10 d)至成熟(花后80 d)期间每2周采集样品,测定可滴定酸含量、pH值、可溶性固形物含量、百粒重、总类胡萝卜素含量、降异戊二烯香气物质含量和类胡萝卜素裂解双加氧酶(CCD)活性,分析其在果实生长期的变化规律,探讨类胡萝卜素含量、CCD活性和降异戊二烯香气物质含量变化的相关性。结果表明,霞多丽葡萄果实生长期共定性定量6种降异戊二烯香气物质,随着果实成熟,降异戊二烯香气物质总含量呈上升趋势;幼果期(花后10~38 d)果实样品CCD活性呈上升趋势,转色期(花后38~52 d)果实样品CCD活性呈下降趋势,成熟期(花后52~80 d)果实样品CCD活性呈上升趋势;总类胡萝卜素含量整体呈下降趋势,但转色期前后会有一个生成积累小高峰;相关性分析显示,CCD活性与总类胡萝卜素含量呈负相关,与游离态和结合态α-紫罗兰酮、β-紫罗兰酮、β-大马士酮以及游离态香叶基丙酮、结合态6-甲基-5-庚烯-2-酮呈正相关。不同生产季样品比较发现,生产季气候对果实理化指标、总类胡萝卜素含量、CCD活性和降异戊二烯香气物质含量均有影响。In order to determine the pattern of production and accumulation for norisoprenoids of Chardonnay grape berries,samples were collected every 2 weeks from young grapes(10 days after flowering)to maturity(80 days after flowering)in two consecutive production seasons in 2020 and 2021.The titratable acidity,pH,soluble solids,100-grain weight,total carotenoids,norisoprenoids and carotenoid cleavage dioxygenase(CCD)enzyme activity were measured,and their change pattern were analyzed during the growth,and the correlation among carotenoids,CCD enzyme activity and norisoprenoids was discussed.The results showed that 6 norisoprenoids were determined during the growth.The total content of norisoprenoids showed an upward trend with the fruit ripened.CCD enzyme activity tended to increase in young fruits(10-38 days after flowering),to decrease in veraison(38-52 days after flowering),and to increase in fruits ripening stage(52-80 days after flowering).The total carotenoid content showed an overall decreasing trend,but there was a small accumulation peak before or after the veraison.The correlation analysis showed that CCD enzyme activity was negatively correlated with total carotenoid content,and positively correlated with free and boundα-ionone,β-ionone,β-damascenone,free geranylacetone,and bound 6-methylhept-5-en-2-one.Comparison of samples from different production seasons found that the climate of the production season had an effect on the physicochemical index,total carotenoid content,CCD enzyme activity and the content of norisoprenoids in the berries.

关 键 词：霞多丽葡萄 类胡萝卜素 类胡萝卜素裂解双加氧酶(CCD) 降异戊二烯 

分 类 号：S663.1[农业科学—果树学]