A“T.E.S.T.”hydrogel bioadhesive assisted by corneal cross-linking for in situ sutureless corneal repair  

作　　者：Meiyan Li Ruoyan Wei Chang Liu Haowei Fang Weiming Yang Yunzhe Wang Yiyong Xian Kunxi Zhang Yong He Xingtao Zhou 

机构地区：[1]Department of Ophthalmology,EYE&ENT Hospital of Fudan University Shanghai,China [2]NHC Key Laboratory of Myopia(Fudan University),Shanghai,China [3]State Key Laboratory of Fluid Power and Mechatronic Systems,School of Mechanical Engineering,Zhejiang University,Hangzhou,China [4]Shanghai Research Center of Ophthalmology and Optometry,Shanghai,China [5]Department of Ophthalmology,Children’s Hospital of Fudan University,National Children’s Medical Center,Shanghai,China [6]Department of Polymer Materials,School of Materials Science and Engineering,Shanghai University,Shanghai,China

出　　处：《Bioactive Materials》2023年第7期333-346,共14页生物活性材料（英文）

基　　金：This work was supported by the Shanghai Rising-Star Program(Grants No.21QA1401500);Clinical Research Plan of SHDC(Grants No.SHDC2020CR1043B).

摘　　要：Corneal transplantation is an effective clinical treatment for corneal diseases,which,however,is limited by donor corneas.It is of great clinical value to develop bioadhesive corneal patches with functions of“Transparency”and“Epithelium&Stroma generation”,as well as“Suturelessness”and“Toughness”.To simultaneously meet the“T.E.S.T.”requirements,a light-curable hydrogel is designed based on methacryloylated gelatin(GelMA),Pluronic F127 diacrylate(F127DA)&Aldehyded Pluronic F127(AF127)co-assembled bi-functional micelles and collagen type I(COL I),combined with clinically applied corneal cross-linking(CXL)technology for repairing damaged cornea.The patch formed after 5 min of ultraviolet irradiation possesses transparent,highly tough,and strongly bio-adhesive performance.Multiple cross-linking makes the patch withstand deformation near 600%and exhibit a burst pressure larger than 400 mmHg,significantly higher than normal intraocular pressure(10-21 mmHg).Besides,the slower degradation than GelMA-F127DA&AF127 hydrogel without COL I makes hydrogel patch stable on stromal beds in vivo,supporting the regrowth of corneal epithelium and stroma.The hydrogel patch can replace deep corneal stromal defects and well bio-integrate into the corneal tissue in rabbit models within 4 weeks,showing great potential in surgeries for keratoconus and other corneal diseases by combining with CXL.

关 键 词：Tough hydrogel Bioadhesives Corneal patch CXL Sutureless repair 

分 类 号：R772.2[医药卫生—眼科]