三相分离法提取裂殖壶藻中油脂、蛋白质和多糖的工艺优化  

Process optimization of three phase partitioning to extract the lipids,protein and polysaccharide from Schizochytrium sp.

作　　者：魏登枭 车丹丹 陈必链[1,2] 何勇锦[1,2,3] 周志华 WEI Dengxiao;CHE Dandan;CHEN Bilian;HE Yongjin;ZHOU Zhihua(Collage of Life Science,Fujian Normal University,Fuzhou 350001,China;Engineering Research Center of Ministry of Education of Industrial Microbiology,Fujian Normal University,Fuzhou 350001,China;Fujian Duopujian Aquatic Technology Co.,Ltd.,Fuzhou 350001,China)

机构地区：[1]福建师范大学生命科学学院,福州350001 [2]福建师范大学工业微生物教育部工程研究中心,福州350001 [3]福建省多普健水产科技有限公司,福州350001

出　　处：《中国油脂》2023年第3期123-129,共7页China Oils and Fats

基　　金：福建省科技厅引导性项目(2020N008)。

摘　　要：为了高值化综合开发裂殖壶藻,以未破壁的裂殖壶藻粉为原料,优化三相分离(TPP)法联产提取裂殖壶藻中油脂、蛋白质和多糖的工艺。结果表明,采用TPP法联产提取裂殖壶藻中油脂、蛋白质和多糖的最佳条件为悬浮液中硫酸铵质量浓度5 g/15 mL,叔丁醇与悬浮液体积比3∶1,液料比120∶1。在上述优化的条件下,TPP法提取裂殖壶藻油脂的最佳提取温度和提取时间分别为90℃和3 h,提取蛋白质和多糖的最佳提取温度和提取时间分别为60℃和0.5 h。此外,评估不同策略下TPP法联产提取裂殖壶藻油脂、蛋白质和多糖的性能发现,先在60℃下提取0.5 h再在90℃下提取3 h,总油脂、蛋白质和多糖提取率最高,分别达97.86%、57.11%和28.15%,油脂中DHA含量可达35.30%,DPA含量可达5.53%。综上,TPP法是一种高效联产提取裂殖壶藻中油脂、蛋白质和多糖的方法。In order to the comprehensive development of Schizochytrium sp.with high value,the process of the simultaneous extraction of oil,protein and polysaccharide from the unbroken Schizochytrium sp.by three phase partitioning(TPP)method were optimized.The results showed that the optimal conditions for the simultaneous extraction of oil,protein and polysaccharide from Schizochytrium sp.by TPP method were as follows:mass concentration of ammonium sulfate in suspension 5 g/15 mL,volume ratio of t-butanol to suspension 3:1,ratio of total volume to algal powder mass 120:1.Under the optimized conditions,the optimal extraction temperature and time of lipids from Schizochytrium sp.by TPP method were 90℃and 3 h,respectively,and the optimal extraction temperature and time of protein and polysaccharide were 60℃and 0.5 h,respectively.Moreover,the extraction performance of TPP method to simultaneously extract the lipids,protein and polysaccharide from Schizochytrium sp.was further evaluated by different strategies.It was found that the extraction at 60℃for 0.5 h followed by 90℃for 3 h resulted in the highest extraction rates of 97.86%,57.11%and 28.15%for total lipids,protein and polysaccharide,respectively,with DHA content up to 35.30%and DPA content up to 5.53%in the lipids.In conclusion,the TPP method is a highly efficient methodfor simultaneously extracting lipids,protein and polysaccharideefrom Schizochytrium sp.

关 键 词：三相分离法 裂殖壶藻 油脂 蛋白质 多糖 

分 类 号：TS222[轻工技术与工程—粮食、油脂及植物蛋白工程] Q939.97[轻工技术与工程—食品科学与工程]