白术多糖基于Wnt/β-catenin信号通路治疗溃疡性结肠炎小鼠的实验研究  

Rhizoma Atractylodes macrocephala polysaccharide acts on ulcerative colitis mice via Wnt/β-catenin signaling pathway

作　　者：陈泰宇[1] 唐学贵[1] 蒋小东 唐诗宇 陈思敏[4] 张智彬 王秋晓 Chen Tai-yu;Tang Xue-gui;Jiang Xiao-dong;Tang Shi-yu;Chen Si-min;Zhang Zhi-bin;Wang Qiu-xiao(Anorectal Department of Integrated Traditional Chinese and Western Medicine,Affiliated Hospital of North Sichuan Medical College,Nanchong,Sichuan 637002,China;Department of Urology,Affiliated Hospital of North Sichuan Medical College,Nanchong,Sichuan 637002,China;Clinical Medical College,North Sichuan Medical College,Nanchong,Sichuan 637000,China;Institute of Anorectal Diseases,North Sichuan Medical College,Nanchong,Sichuan 637000,China;Department of Clinical Medicine of Traditional Chinese and Western Medicine,North Sichuan Medical College,Nanchong,Sichuan 637000,China)

机构地区：[1]川北医学院附属医院中西医结合肛肠科,四川南充637002 [2]川北医学院附属医院泌尿外科,四川南充637002 [3]川北医学院临床医学院,四川南充637000 [4]川北医学院肛肠疾病研究所,四川南充637000 [5]川北医学院中西医临床医学系,四川南充637000

出　　处：《中国现代医学杂志》2023年第8期24-30,共7页China Journal of Modern Medicine

基　　金：国家自然基金面上项目(No:82074429);南充市科技局市校科技战略合作专项(No:22SXQT0076)。

摘　　要：目的探究白术多糖基于Wnt/β-catenin信号通路对溃疡性结肠炎(UC)小鼠的影响。方法将40只小鼠随机分为对照组、模型组、西药组和白术多糖组,每组10只。使用葡聚糖硫酸钠盐(DSS)诱导UC,西药组、白术多糖组分别使用美沙拉嗪、白术多糖灌胃。记录小鼠体质量、小肠推进率及结肠组织病理学评分。Western blotting检测Wnt1、β-catenin、C-MYC、细胞周期素D1(CyclinD1)蛋白的表达;ELISA检测血清肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-1β(IL-1β)、胃动素(MTL)、胃泌素(GAS)水平;流式细胞术检测调节性T细胞(Treg)、辅助性T细胞1(Th1)、辅助性T细胞2(Th2)、辅助性T细胞17(Th17)细胞百分比。结果4组小鼠实验前及实验第1、2、3周的体质量比较,结果:①不同时间点体质量有差异(F=7.442,P=0.001);②4组体质量有差异(F=35.614,P=0.000),对照组、西药组和白术多糖组体质量高于模型组;③4组体质量变化趋势有差异(F=22.375,P=0.000)。模型组组织病理学评分高于对照组、西药组和白术多糖组(P<0.05),小肠推进率低于对照组、西药组和白术多糖组(P<0.05)。对照组Wnt1、β-catenin、C-MYC、CyclinD1蛋白相对表达量、TNF-α、IL-6、IL-1β水平及Th1、Th2、Th17细胞百分比低于模型组、西药组、白术多糖组(P<0.05),MTL、GAS水平、Treg细胞百分比高于模型组、西药组和白术多糖组(P<0.05);模型组Wnt1、β-catenin、C-MYC、CyclinD1蛋白相对表达量,TNF-α、IL-6、IL-1β水平,Th1、Th2、Th17细胞百分比高于西药组、白术多糖组(P<0.05),MTL、GAS水平及Treg细胞百分比低于西药组和白术多糖组(P<0.05)。结论白术多糖能够抑制DSS诱导的UC,其机制可能是通过下调Wnt/β-catenin信号通路,调节免疫功能,减轻炎症反应,促进受损肠道黏膜修复实现的。Objective To investigate the effect of Rhizoma Atractylodes macrocephala polysaccharide on ulcerative colitis(UC)mice by highlighting the Wnt/β-catenin signaling pathway.Methods Forty mice were randomly divided into control group,model group,Western medicine group and Rhizoma Atractylodes macrocephala polysaccharide group,with 10 mice in each group.UC was induced by dextran sodium sulfate(DSS),and mesalazine and Rhizoma Atractylodes macrocephala polysaccharide were administered by gavage to mice in the Western medicine group and Rhizoma Atractylodes macrocephala polysaccharide group,respectively.The body mass,intestinal propulsion rate and colon histopathological score were recorded.The expressions of Wnt1,β-catenin,C MYC and cyclin D1 were detected via Western blotting.Serum levels of tumor necrosis factorα(TNFα),interleukin-6(IL-6),interleukin-1β(IL-1β),motilin(MTL),and gastrin(GAS)were determined via ELISA.The percentages of regulatory T cells(Treg),T helper 1 cells(Th1),T helper 2 cells(Th2),and T helper 17 cells(Th17)were detected.Results The body mass of mice in the four groups before and 1 week,2 weeks and 3 weeks after the experiment were compared,and the results revealed that the body mass was different among the time points(F=7.442,P=0.001)and the groups(F=35.614,P=0.000),and that the body mass in the control group,Western medicine group and Rhizoma Atractylodes macrocephala polysaccharide group was higher than that in the model group.Besides,the change trends of the body mass were different among the four groups(F=22.375,P=0.000).The histopathological score of the model group was higher than that of the control group,the Western medicine group and the Rhizoma Atractylodes macrocephala polysaccharide group(P<0.05),while the intestinal propulsion rate of the model group was lower than that of the control group,the Western medicine group and the Rhizoma Atractylodes macrocephala polysaccharide group(P<0.05).The protein expressions of Wnt1,β-catenin,C-MYC and cyclin D1,serum levels of TNF-α,IL-6 an

关 键 词：溃疡性结肠炎 WNT/Β-CATENIN信号通路 白术多糖 小鼠 

分 类 号：R574.62[医药卫生—消化系统]