A multifunctional neuromodulation platform utilizing Schwann cell-derived exosomes orchestrates bone microenvironment via immunomodulation, angiogenesis and osteogenesis  

作　　者：Zhichao Hao Lin Ren Zhen Zhang Zaiwu Yang Shujie Wu Gen Liu Bin Cheng Jun Wu Juan Xia 

机构地区：[1]Hospital of Stomatology,Sun Yat-sen University,Guanghua School of Stomatology,Sun Yat-sen University,and Guangdong Provincial Key Laboratory of Stomatology,Guangzhou,510055,China [2]School of Biomedical Engineering,Shenzhen Campus of Sun Yat-sen University,Shenzhen,518107,China [3]Bioscience and Biomedical Engineering Thrust,The Hong Kong University of Science and Technology(Guangzhou),Nansha,Guangzhou,511400,China [4]Division of Life Science,The Hong Kong University of Science and Technology,999077,Hong Kong,China

出　　处：《Bioactive Materials》2023年第5期206-222,共17页生物活性材料（英文）

基　　金：This work was supported by National Natural Science Foundation of China.(No.82170960,81870769,51973243,52173150 and 82201098);Guangdong Basic and Applied Basic Research Foundation(No.2019A1515110379 and 2021A1515010782);the Shenzhen Basic Research Project(JCYJ20190807155801657).

摘　　要：Recent evidence highlights multifaceted biological needs to recapitulate the bone microenvironment for bone regeneration.Neurotization has great potential for realizing multi-system modulations in bone tissue engineering(BTE).However,a neural strategy involving all the key bone repair steps temporally has not yet been reported.In this study,we reported the neural tissue engineering hydrogel-encapsulated Schwann cell-derived exosomes(SC Exo).This sustained-release SC Exo system prominently enhanced bone regeneration by promoting innervation,immunoregulation,vascularization,and osteogenesis in vivo.Moreover,the in vitro results further confirmed that this system significantly induced M2 polarization of macrophages,tube formation of HUVECs,and BMSCs osteogenic differentiation.Furthermore,BMSCs osteogenesis was promoted by upregulating the TGF-β1/SMAD2/3 signaling pathway.In summary,a novel cell-free and easily prepared SC Exo neural engineering was successfully developed to promote bone regeneration by orchestrating the entire bone healing microenvironment,which may provide a new strategy for tissue engineering and clinical treatment of bone defects.

关 键 词：Bone microenvironment Bone regeneration Schwann cells EXOSOMES Neurotization in bone tissue engineering 

分 类 号：R318.08[医药卫生—生物医学工程]