针康法对合并2型糖尿病的VaD大鼠认知功能的影响及其机制  

Effect of acupuncture-rehabilitation therapy on cognitive function of VaD rats with type 2 diabetes mellitus and its mechanism

作　　者：唐强[1] 樊宇婷 朱路文[1,2] 梁碧莹 任婷 于国强 关莹 TANG Qiang;FAN Yuting;ZHU Luwen;LIANG Biying;REN Ting;YU Guoqiang;GUAN Ying(Department of Rehabilitation,The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150001,China;不详)

机构地区：[1]黑龙江中医药大学附属第二医院康复科,哈尔滨150001 [2]黑龙江中医药大学研究生院 [3]黑龙江省中医药科学院研究生院

出　　处：《山东医药》2023年第13期28-33,共6页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(82004439);黑龙江省中医药科研项目(GY2022-19);黑龙江省孙申田人才基金项目(2019KY-06)。

摘　　要：目的探讨针康法对合并2型糖尿病(T2DM)的血管性痴呆(VaD)大鼠认知功能的影响及其机制。方法选择健康雄性SD大鼠75只,通过腹腔注射链脲佐菌素制备T2DM模型,随机选取其中12只作为假手术组,剩余63只继续制备VaD模型。63只制备VaD模型大鼠中,模型制备成功48只,随机分为模型组、跑台训练组、头穴丛刺组、针康组,每组12只。VaD模型制备结束第5天,针康组予头穴丛刺治疗,留针期间予跑台训练,头穴丛刺组仅予头穴丛刺治疗,跑台训练组仅予跑台训练。假手术组和模型组不予任何治疗。每组干预14、28天随机取6只,干预14天须连续治疗14天,干预28天须连续治疗28天。各组干预第14、28天结束行Morris水迷宫实验,检测学习记忆功能。Morris水迷宫实验结束,腹腔注射过量麻醉处死,断头取脑,取一侧海马组织,采用ELISA法检测氧化应激相关指标超氧化物歧化酶(SOD)、丙二醛(MDA)、还原型谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-Px)以及胆碱能神经递质相关指标乙酰胆碱(Ach)、乙酰胆碱酯酶(AchE)含量;取另一侧海马组织,Nissl染色,观察海马组织神经元形态变化。结果模型组干预14、28天逃避潜伏期均显著高于假手术组同期,穿越平台次数、目标象限停留时间百分比均显著低于假手术组同期(P均<0.05);跑台训练组、头穴丛刺组、针康组干预14、28天逃避潜伏期均显著低于模型组同期,穿越平台次数、目标象限停留时间百分比均显著高于模型组同期(P均<0.05);针康组干预14、28天逃避潜伏期均显著低于跑台训练组、头穴丛刺组同期,穿越平台次数、目标象限停留时间百分比均显著高于跑台训练组、头穴丛刺组同期(P均<0.05);跑台训练组、头穴丛刺组、针康组干预14天逃避潜伏期均显著高于干预28天,穿越平台次数、目标象限停留时间百分比均显著低于干预28天(P均<0.05)。模型组干预14、28天海马Objective To investigate the effect of acupuncture-rehabilitation therapy on cognitive function of vascu⁃lar dementia(VaD)rats with type 2 diabetes mellitus(T2DM)and its mechanism.Methods Seventy-five healthy male SD rats were selected to establish T2DM models by peritoneal injection of streptozotocin.Twelve of them were randomly selected as the sham operation group,and the remaining 63 rats were continued to prepare VaD models.Among 63 VaD model rats,48 were successfully established and randomly divided into the model group,treadmill training group,scalp acupuncture group and acupuncture-rehabilitation group,with 12 rats in each group.On the fifth day after the end of VaD preparation,rats in the acupuncture-rehabilitation group were given scalp acupuncture,and received treadmill training during needle retention;rats in the scalp acupuncture group were only treated with scalp acupuncture,and rats in the treadmill training group only received treadmill training.The rats in the sham operation group and the model group were not treated.Each group was randomly divided into 14 and 28 days of intervention,and we selected 6 rats at each time point;14 days of intervention required 14 days of continuous treatment and 28 days of intervention required 28 days of con⁃tinuous treatment.Morris water maze test was performed on the 14th and 28th days of intervention to detect learning and memory function.After the Morris water maze experiment,we injected excessive anesthesia into the abdominal cavity to kill the rats and decapitated to obtain their brains.The levels of superoxide orgotein dismutase(SOD),maleic dialdehyde(MDA),glutathione(GSH)and glutathione peroxidase(GSH-Px),which were related to oxidative stress,and acetylcho⁃line(Ach)and acetylcholinesterase(AchE),which were related to cholinergic neurotransmitters,were determined by ELISA in one side of the hippocampus tissues.Nissl staining was performed on the other side of hippocampus tissues to observe the morphological changes of hippocampal neurons.Results The es

关 键 词：血管性痴呆 2型糖尿病 针康法 氧化应激 胆碱能神经递质 大鼠 

分 类 号：R741[医药卫生—神经病学与精神病学] R587.1[医药卫生—临床医学]