独角莲醇提物对胰腺癌细胞恶性生物学行为及上皮间质转化的影响  

Effects of alcohol extracts of Typhonium giganteum on malignant biological behaviors and epithelial-mesenchymal transition of pancreatic cancer cells

作　　者：高合 彭青侠 许小凡[1] 范建伟 段丽芳 张红[1,3] GAO He;PENG Qingxia;XU Xiaofan;FAN Jianwei;DUAN Lifang;ZHANG Hong(College of Basic Medicine,Shaanxi University of Chinese Medicine,Xianyang 712000,China;不详)

机构地区：[1]陕西中医药大学基础医学院,陕西咸阳712000 [2]咸阳市三原县医院儿科 [3]陕西省免疫炎症相关疾病中医药防治国际联合研究中心

出　　处：《山东医药》2023年第13期40-43,共4页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(82174201、82104815);陕西中医药大学学科创新团队建设项目(2019-YL14)。

摘　　要：目的探讨独角莲醇提物对胰腺癌细胞恶性生物学行为及上皮间质转化(EMT)的影响。方法体外培养人胰腺癌细胞PANC-1,取传3代、对数生长期、生长状态良好的PANC-1细胞,随机分为Control组及独角莲醇提物500、600、700、800、900μg/mL组,独角莲醇提物500、600、700、800、900μg/mL组分别予相应浓度的独角莲醇提物干预,Control组不予独角莲醇提物干预。干预24、48 h,采用CCK-8法检测细胞活力;干预48 h,采用划痕实验检测细胞迁移能力,采用流式细胞术检测细胞凋亡率,采用Western blotting法检测凋亡相关蛋白Bax、Bcl-2及EMT相关蛋白E-cadherin、N-cadherin表达。结果与Control组比较,独角莲醇提物500μg/mL组培养24、48 h细胞活力均显著升高,而独角莲醇提物600、700、800、900μg/mL组培养24、48 h细胞活力均显著降低(P均<0.05)。随着浓度增加和培养时间延长,独角莲醇提物各浓度组细胞活力显著下降,呈现浓度和时间依赖性(P均<0.05)。独角莲醇提物500μg/mL组无论是培养24 h还是培养48 h细胞活力均显著升高,在后续研究中剔除。此外,由于培养48 h独角莲醇提物各浓度组细胞活力降低更显著,故选择培养48 h进行后续研究。与Control组比较,独角莲醇提物600、700、800、900μg/mL组划痕愈合率逐渐降低,细胞凋亡率逐渐升高,凋亡相关蛋白Bcl-2表达下调、Bax表达上调,EMT相关蛋白E-cadherin表达上调、N-cadherin表达下调(P均<0.05)。结论一定浓度的独角莲醇提物不仅能抑制人胰腺癌细胞增殖和迁移并促进其凋亡,还能抑制其EMT。Objective To investigate the effects of ethanol extracts of Typhonium giganteum on malignant characteris⁃tic behaviors and epithelial-mesenchymal transition(EMT)of pancreatic cancer cells.Methods The human pancreatic cancer cell PANC-1 was cultured in vitro.PANC-1 cell in the third passage,which were in logarithmic growth phase and had good growth status,were randomly divided into the Control group and 500,600,700,800 and 900μg/mL ethanol extracts of Typhonium giganteum groups.PANC-1 cells in the 500,600,700,800 and 900μg/mL ethanol extracts of Typhonium giganteum groups were intervened with the ethanol extracts of Typhonium giganteum at corresponding concentra⁃tions,while PANC-1 cells in the Control group were not intervened with the ethanol extracts of Typhonium giganteum.After 24 and 48 h of intervention,the cell viability was detected by CCK-8 assay,cell migration ability was detected by Scratch test,and apoptosis rate was detected by flow cytometry.The expression levels of apoptosis-related proteins(Bax,Bcl-2)and EMT-related proteins(E-cadherin and N-cadherin)were detected by Western blotting.Results Compared with the Control group,the cell viability in the 500μg/mL ethanol extract of Typhonium giganteum group significantly increased at 24 and 48 h,while those in the 600,700,800 and 900μg/mL ethanol extracts of Typhonium giganteum groups significantly decreased at 24 and 48 h(all P<0.05).With the increase of concentrations and the extension of culture time,the cell viability of ethanol extracts of Typhonium giganteum groups decreased significantly,in a concentration-and time-dependent manner(P<0.05).The cell viability of the 500μg/mL ethanol extract of Typhonium giganteum group significantly increased regardless of 24 h or 48 h of culture,which was excluded in the subsequent study.In addition,because the cell via⁃bility of ethanol extract of Typhonium giganteum groups at different concentrations decreased more significantly after 48 h of culture,48 h culture was selected for follow-up study.Compar

关 键 词：胰腺癌 独角莲醇提物 恶性生物学行为 上皮间质转化 

分 类 号：R735.9[医药卫生—肿瘤]