Preconditioning process for dermal tissue decellularization using electroporation with sonication  

作　　者：Mi Hee Leeand Jong-Chul Park Min-Ah Koo HaKyeong Jeong Seung Hee Hong Gyeung Mi Seon Mi Hee Lee Jong-Chul Park 

机构地区：[1]Cellbiocontrol Laboratory,Yonsei University College of Medicine,50-1 Yonsei-ro,Seodaemun-gu,Seoul 03722,Republic of Korea [2]Department of Medical Engineering,Graduate School of Medical Science,Brain Korea 21 Project,Yonsei University College of Medicine,50-1 Yonsei-ro,Seodaemun-gu,Seoul 03722,Republic of Korea

出　　处：《Regenerative Biomaterials》2022年第1期22-29,共8页再生生物材料（英文版）

基　　金：supported by the National Research Foundation of Korea(NRF)grant funded by the Korea government(MSIT)(No.2017M3A9B3063638,No.2019R1A2C2005256).

摘　　要：Decellularization to produce bioscaffolds composed of the extracellular matrix(ECM)uses enzymatic,chemical and physical methods to remove antigens and cellular components from tissues.Effective decellularization methods depend on the characteristics of tissues,and in particular,tissues with dense,complex structure and abundant lipid content are difficult to completely decellularize.Our study enables future research on the development of methods and treatments for fabricating bioscaffolds via decellularization of complex and rigid skin tissues,which are not commonly considered for decellularization to date as their structural and functional characteristics could not be preserved after severe decellularization.In this study,decellularization of human dermal tissue was done by a combination of both chemical(0.05%trypsin-EDTA,2%SDS and 1%Triton X-100)and physical methods(electroporation and sonication).After decellularization,the content of DNA remaining in the tissue was quantitatively confirmed,and the structural change of the tissue and the retention and distribution of ECM components were evaluated through histological and histochemical analysis,respectively.Conditions of the chemical pretreatment that increase the efficiency of physical stimulation as well as decellularization,and conditions for electroporation and sonication without the use of detergents,unlike the methods performed in previous studies,were established to enable the complete decellularization of the skin tissue.The combinatorial decellularization treatment formed micropores in the lipid bilayers of the skin tissues while removing all cell and cellular residues without affecting the ECM properties.Therefore,this procedure can be widely used to fabricate bioscaffolds by decellularizing biological tissues with dense and complex structures.

关 键 词：DECELLULARIZATION ELECTROPORATION sonication extracellular matrix 

分 类 号：R318[医药卫生—生物医学工程]