Fabrication and biological evaluation of 3D-printed calcium phosphate ceramic scaffolds with distinct macroporous geometries through digital light processing technology  

作　　者：Jing Wang Yitao Tang Quanle Cao Yonghao Wu Yitian Wang Bo Yuan Xiangfeng Li Yong Zhou Xuening Chen Xiangdong Zhu Chongqi Tu Xingdong Zhang 

机构地区：[1]National Engineering Research Center for Biomaterials,College of Biomedical Engineering,Sichuan University,Chengdu 610064,China [2]Department of Orthopaedics,West China Hospital of Sichuan University,Chengdu 610041,China

出　　处：《Regenerative Biomaterials》2022年第1期389-403,共15页再生生物材料（英文版）

基　　金：sponsored by the National Key Research and Development Program of China(2017YFB0702600);National Natural Science Foundation of China(31971283,31670985);Sichuan Science and Technology Programs(2019JDTD0008,2021YFS0032).

摘　　要：Digital light processing(DLP)-based 3D printing technique holds promise in fabricating scaffolds with high precision.Here raw calcium phosphate(CaP)powders were modified by 5.5%monoalcohol ethoxylate phosphate(MAEP)to ensure high solid loading and low viscosity.The rheological tests found that photocurable slurries composed of 50wt%modified CaP powders and 2wt%toners were suitable for DLP printing.Based on geometric models designed by computer-aided design(CAD)system,three printed CaP ceramics with distinct macroporous structures were prepared,including simple cube,octet-truss and inverse face-centered cube(fcc),which presented the similar phase composition and microstructure,but the different macropore geometries.Inverse fcc group showed the highest porosity and compressive strength.The in vitro and in vivo biological evaluations were performed to compare the bioactivity of three printed CaP ceramics,and the traditional foamed ceramic was used as control.It suggested that all CaP ceramics exhibited good biocompatibility,as evidence by an even bone-like apatite layer formation on the surface,and the good cell proliferation and spreading.A mouse intramuscular implantation model found that all of CaP ceramics could induce ectopic bone formation,and foam group had the strongest osteoinduction,followed by inverse fcc,while cube and octet-truss had the weakest one.It indicated that macropore geometry was of great importance to affect the osteoinductivity of scaffolds,and spherical,concave macropores facilitated osteogenesis.These findings provide a strategy to design and fabricate high-performance orthopedic grafts with proper pore geometry and desired biological performance via DLP-based 3D printing technique.

关 键 词：digital light processing(DLP) 3D printing pore structure OSTEOINDUCTION 

分 类 号：R318[医药卫生—生物医学工程]