Three-dimensional bioprinting sodium alginate/gelatin scaffold combined with neural stem cells and oligodendrocytes markedly promoting nerve regeneration after spinal cord injury  

作　　者：Shuo Liu Hui Yang Dong Chen Yuanyuan Xie ChenXu Tai Liudi Wang Peng Wang Bin Wang 

机构地区：[1]Clinical Stem Cell Center,Nanjing Drum Tower Hospital,the Affiliated Hospital of Nanjing University Medical School,Nanjing 210000,Jiangsu Province,China [2]State Key Laboratory of Pharmaceutical Biotechnology,Department of Sports Medicine and Adult Reconstructive Surgery,Nanjing Drum Tower Hospital,The Affiliated Hospital of Nanjing University Medical School,Nanjing 210000,Jiangsu Province,China

出　　处：《Regenerative Biomaterials》2022年第1期522-532,共11页再生生物材料（英文版）

基　　金：supported by following grants:the National Key Research and Development Program of China(grant number 2017YFA0104304);the National Natural Science Foundation of China(grant numbers 81571213,82070459 to B.W.,grant numbers 81800583 to Y.Y.X.);Key Project of Jiangsu Province(grant number BE2020765 to B.W.);Nanjing Medical Science and Technique Development Foundation(grant numbers QRX17006,QRX17057,ZKX20016 to B.W.);Nanjing Medical Science and Technique Development Foundation(grant number YKK20071 to H.Y.);Jiangsu Provincial Plan for Mass Entrepreneurship and Innovation(2019,B.W.);Project of Modern Hospital Management and Development Institute,Nanjing University/Aid project of Nanjing Drum Tower Hospital Health,Education&Research Foundation(grant number NDYG2020030 to B.W.).

摘　　要：Accumulating research has indicated that the transplantation of combined stem cells and scaffolds is an effective method for spinal cord injury(SCI).The development of three-dimensional(3D)bioprinting technology can make the 3D scaffolds combined with cells more accurate and effective for SCI treatment.However,unmyelinated newborn nerve fibers have no nerve signaling conduction,hampering recovery of motor function.In this study,we designed and printed a type of sodium alginate/gelatin scaffold loaded with neural stem cells and oligodendrocytes,which were involved in the formation of the myelin sheaths of neural cell axons.In order to observe the effectiveness of this 3D bioprinting scaffold,we transplanted it into the completely transected rat spinal cord,and then immunofluorescence staining,hematoxylin–eosin staining and behavioral assessment were performed.The results showed that this 3D bioprinting scaffold markedly improved the hindlimb motor function and promoted nerve regeneration.These findings suggested that this novel 3D bioprinting scaffold was a good carrier for cells transplantation,thereby enhancing spinal cord repair following injury.

关 键 词：three-dimensional bioprinting neural stem cell OLIGODENDROCYTES spinal cord injury 

分 类 号：R651.2[医药卫生—外科学] R318[医药卫生—临床医学]