Semi-3D cultures using Laminin 221 as a coating material for human induced pluripotent stem cells  

作　　者：Yoshiki Nakashima Shinsuke Yoshida Masayoshi Tsukahara 

机构地区：[1]Kyoto University Center for iPS Cell Research and Application Foundation(CiRA Foundation),Facility for iPS Cell Therapy(FiT),Kyoto 606-8397,Japan

出　　处：《Regenerative Biomaterials》2022年第1期775-793,共19页再生生物材料（英文版）

基　　金：supported by Japan Agency for Medical Research and Development(AMED)under grant number JP22bm0104001.

摘　　要：It was previously believed that human induced pluripotent stem cells(hiPSCs)did not show adhesion to the coating material Laminin 221,which is known to have specific affinity for cardiomyocytes.In this study,we report that human mononuclear cell-derived hiPSCs,established with Sendai virus vector,form peninsular-like colonies rather than embryonic stem cell-like colonies;these peninsular-like colonies can be passaged more than 10 times after establishment.Additionally,initializationdeficient cells with residual Sendai virus vector adhered to the coating material Laminin 511 but not to Laminin 221.Therefore,the expression of undifferentiated markers tended to be higher in hiPSCs established on Laminin 221 than on Laminin 511.On Laminin 221,hiPSCs15M66 showed a semi-floating colony morphology.The expression of various markers of cell polarity was significantly lower in hiPSCs cultured on Laminin 221 than in hiPSCs cultured on Laminin 511.Furthermore,201B7 and 15M66 hiPSCs showed 3D cardiomyocyte differentiation on Laminin 221.Thus,the coating material Laminin 221 provides semi-floating culture conditions for the establishment,culture and induced differentiation of hiPSCs.

关 键 词：human induced pluripotent stem cells(hiPSCs) regenerative medicine coating material CARDIOMYOCYTE therapeutic cells 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]