Screening and validation of nickel ion cytotoxicity biomarkers based on transcriptomic and proteomic technology  

作　　者：Fudan Zhang Yan Huang Yajing Zhang Xiaoying Lü 

机构地区：[1]State Key Laboratory of Bioelectronics,School of Biological Science and Medical Engineering,Southeast University,Nanjing 210096,China

出　　处：《Regenerative Biomaterials》2022年第1期794-805,共12页再生生物材料（英文版）

基　　金：supported by the National Natural Science Foundation of China(31971254).

摘　　要：The aimof this study was to screen cytotoxicity biomarkers of nickel ions(Ni^(2+))using transcriptomic and proteomic approaches combined with molecular biology validation.First,the MTT method was used to evaluate cytotoxicity in L929 cells treated with Ni^(2+)at different concentrations.Ni^(2+)at both 100μM and 200 lMaffected cell proliferation.Then,transcriptomic and proteomic technology was used to study the effects of Ni^(2+)on the expression of genes/proteins in cells.It was found that 1490,789,652 and 729 genes(12,24,48 and 72 h,respectively)and 177,2191 and 2095 proteins(12,24 and 48 h,respectively)were differentially expressed after treatment with 100μM Ni^(2+).In total,1403,963,916 and 1230 genes(12,24,48 and 72 h,respectively)and 83,1681 and 2398 proteins(12,24 and 48 h,respectively)were differentially expressed after treatment with 200μMNi^(2+).Then,four target gene/protein biomarkers were filtered by combined screening using gene/proteomic experimental data and biological pathway analyses.Further expression level validation of all these target biomarkers and functional validation of selected gene/protein biomarkers were carried out,and a final gene/protein biomarker(UQCRB)was identified.

关 键 词：nickel ion(Ni^(2+)) cytotoxicity biomarkers transcriptomic/proteomic BIOINFORMATICS 

分 类 号：R318[医药卫生—生物医学工程]