淋病奈瑟菌感染小鼠脾脏单个核细胞对NLRP3、IL-1β表达的影响  

Effect of Neisseria gonorrhoeae infection on NLRP3 and IL-1β expression in spleen mononuclear cells of mice

作　　者：魏娜 张方 李街青 陈佳玲 史建强 陈嵘祎 WEI Na;ZHANG Fang;LI Jie-qing;CHEN Jia-ling;SHI Jian-qiang;CHEN Rong-yi(Dermatology Hospital,Southern Medical University,Guangzhou 510091,China;Department of Dermatology,Affiliated Hospital of Guangdong Medical University,Zhanjiang 524001,China)

机构地区：[1]南方医科大学皮肤病医院,广东广州510091 [2]广东医科大学附属医院,广东湛江524001

出　　处：《广东医科大学学报》2023年第2期132-136,共5页Journal of Guangdong Medical University

基　　金：广东省科技创新战略专项基金(2021A1515011218);广州市科技计划项目(202002030106);国家自然科学基金(81872532)。

摘　　要：目的研究淋病奈瑟菌(Ng)感染小鼠脾脏单个核细胞对NLRP3、IL-1β表达的影响。方法通过测定不同稀释度Ng菌液OD450值,结合平板菌落计数法,建立淋病奈瑟菌OD450值(x)-活菌数(y)回归方程。不同浓度Ng悬液感染小鼠脾脏单个核细胞,采用实时荧光定量PCR(qRT-PCR)、Western blot检测NLRP3、IL-1β水平。结果成功建立了淋病奈瑟菌OD450值(x)-活菌数(y)回归方程:y=287.65x-30.583。qRT-PCR、Western blot结果显示,以OD450值=0.2 Ng悬液感染单个核细胞1 h,NLRP3、IL-1β表达量最高。结论利用OD值计数Ng简便、快捷;NLRP3、IL-1β在Ng感染中起关键作用。Objective To investigate the influence of Neisseria gonorrhoeae(Ng)infection on NLRP3 and IL-1β expression in spleen mononuclear cells of mice.Methods The OD450 value(x)-viable count(y)regression equation of Ng was established by OD value measurement of different Ng dilutions and plate colony counting.The spleen mononuclear cells of mice were infected with different Ng concentrations,and their expression NLRP3 and IL-1β was detected by qRT-PCR and Western blot.Results The OD450 value(x)-viable count(y)regression equation of Ng was established:y=287.65x-30.583.qRT-PCR and Western blot showed that NLRP3 and IL-1β expression reached the top in mononuclear cells infected with OD450 value=0.2 Ng suspension for 1 h.Conclusion OD450 value is simple and rapid for Ng counting.NLRP3 and IL-1β play a key role in Ng infection.

关 键 词：淋病奈瑟菌 小鼠 脾脏 NLRP3 IL-1Β 

分 类 号：R575[医药卫生—消化系统]