结肠癌叉头框蛋白P3、促泛素结合衔接子p62及核因子-κB与癌旁肿瘤沉积形成相关性分析  

Expression of Foxp3 p62 and nuclear factor-κB in patients with colon cancer and their correlation with the formation of adjacent tumor deposits

作　　者：胡晓峰[1,2] 武雪亮[1,2] 杨东东[1,2] 王一飞[1,2] Hu Xiaofeng;Wu Xueliang;Yang Dongdong;Wang Yifei(Department of General Surgery,The First Affiliated Hospital of Hebei North University,Cancer Institute,The First Affiliated Hospital of Hebei North University,Zhangjiakou 075000,Hebei,China)

机构地区：[1]河北北方学院附属第一医院普通外科 [2]河北北方学院附属第一医院肿瘤研究所,河北张家口075000

出　　处：《肿瘤代谢与营养电子杂志》2023年第2期219-224,共6页Electronic Journal of Metabolism and Nutrition of Cancer

基　　金：河北省科技厅重点专项(22377784D);河北省卫健委医学发展重点课题计划(20200523)。

摘　　要：目的探究结肠癌患者叉头框蛋白P3(Foxp3)、促泛素结合衔接子(p62)及核因子κB(NF-κB)表达及与癌旁肿瘤沉积形成相关性。方法选取河北北方学院附属第一医院普通外科2019年1月至2022年1月收治的结肠癌患者80例,根据其是否合并癌旁肿瘤沉积分为对照组(36例),沉积组(44例)。使用实时荧光定量PCR技术(qRT-PCR)检测患者Foxp3、p62、NF-κB mRNA表达,使用免疫组化检测患者Foxp3、p62、NF-κB阳性细胞表达,使用蛋白质印迹法检测两组患者病灶组织中Foxp3、p62、NF-κB mRNA相对蛋白表达量。并使用多因素Logistic回归分析以上指标与患者癌旁肿瘤沉积形成相关性。结果比较两组基线资料,差异无统计学意义。沉积组Foxp3、p62、NF-κB mRNA表达显著高于对照组,差异具有统计学意义(P<0.05);沉积组Foxp3、p62、NF-κB免疫组化阳性细胞占比表达显著高于对照组,差异具有统计学意义(P<0.05);沉积组Foxp3、p62、NF-κB蛋白相对表达显著高于对照组,差异具有统计学意义(P<0.05);多因素Logistic回归分析结果显示,Foxp3、p62、NF-κB mRNA,Foxp3、p62、NF-κB阳性细胞占比,Foxp3、p62、NF-κB蛋白相对表达均与患者癌旁肿瘤沉积发生具有显著相关性,其高表达均是患者发生癌旁肿瘤沉积的独立危险因素(P<0.05)。结论合并癌旁肿瘤沉积结肠癌患者病灶Foxp3、p62、NF-κB mRNA,Foxp3、p62、NF-κB阳性细胞占比及Foxp3、p62、NF-κB蛋白相对表达均升高,且其高水平表达是结直肠癌患者癌旁肿瘤沉积形成独立危险因素,在患者实际治疗中,应重视以上指标表达,有助于评估及预测患者病情进展。Objective To explore the expression of Foxp3,p62 and NF-κB in patients with colon cancer and their correlation with the formation of adjacent tumor deposits.Method The research samples were collected from 80 colon cancer patients admitted to Department of General Surgery The First Affiliated Hospital of Hebei North University from January 2019 to January 2022,and were divided into control group(36 cases)and deposition group(44 cases)according to whether they had adjacent tumor deposits.The expression of Foxp3,p62 and NF-KB mRNA in patients was detected by qRT-PCR,the expression of Foxp3,p62 and NF-KB positive cells in patients was detected by immunohistochemistry,and Foxp3,p62,NF-κB in the lesion organizers of the two groups of patients were detected by western bltting experiment.NF-κB mRNA relative protein expression.Logistic multivariate regression was used to analyze the correlation between the above indicators and the formation of paracancerous tumor deposits in patients.Result The mRNA expressions of Foxp3,p62 and NF-κB in the deposition group were significantly higher than those in the control group,and the difference was statistically significant(P<0.05).The difference was statistically significant in the control group(P<0.05);the relative expressions of Foxp3,p62,and NF-κB proteins in the deposition group were significantly higher than those in the control group,and the difference was statistically significant(P<0.05);Logistic multivariate regression analysis showed that,Foxp3,p62,NF-κB mRNA,the proportion of Foxp3,p62,NF-κB positive cells,and the relative expression of Foxp3,p62,NF-κB protein were all significantly correlated with the occurrence of adjacent tumor deposits in patients.The independent risk factors in patients with adjacent tumor deposits(P<0.05).Conclusion The Foxp3,p62,NF-κB mRNA,the proportion of Foxp3,p62,NF-κB positive cells and the proportion of Foxp3,p62 and NF-κB positive cells in colon cancer patients with adjacent tumor deposits The relativeexpressions of p62 and NF-κB

关 键 词：结肠癌 肿瘤沉积 叉头框蛋白 p62蛋白 核因子ΚB 

分 类 号：R735.35[医药卫生—肿瘤]