微波辅助酸水解-HPLC测定紫肉甘薯中矢车菊素和芍药素的含量  

Determination of Cyanidin and Peonidin in Purple Sweet Potato by Microwave-Assisted Acid Hydrolysis-High Performance Liquid Chromatography

作　　者：孙皓熠 孙敬勇[1] 姚庆强[1] SUN Hao-yi;SUN Jing-yong;YAO Qing-qiang(School of Parmacy and Pharmaceutical Sciences&Institute of Materia Medica,Shandong First Medical University&Shandong Academy of Medical Sciences,Jinan 250117,China)

机构地区：[1]山东第一医科大学药学与制药科学学院,山东省医学科学院药物研究所,山东济南250117

出　　处：《食品与药品》2023年第2期149-152,共4页Food and Drug

基　　金：山东省自然科学基金项目(编号:ZR2020MC032)。

摘　　要：目的建立微波水解-高效液相色谱(HPLC)法同时测定紫肉甘薯中矢车菊素和芍药素两个花青素成分含量的方法。方法样品经盐酸甲醇(3→10)微波水解后,采用Agilent Eclipse XDB-C_(18)(250 mm×4.6 mm,5μm)色谱柱,以乙腈-0.1%三氟乙酸水溶液(80:20)为流动相,流速1.0 ml/min,检测波长538 nm,柱温25℃,进样量10μl。结果矢车菊素和芍药素进样量分别在0.045~0.225μg(r=0.9998)和0.102~0.510μg(r=0.9997)范围内线性关系良好。两者平均回收率分别为98.6%,RSD=0.88%(n=6)和98.8%,RSD=1.2%(n=6)。结论该方法简便、准确可靠、重复性强,可作为同时测定紫肉甘薯中矢车菊素和芍药素含量的方法。Objective To develop a method for simultaneous determination of cyanidin and peonidin in purple sweet potato by microwave-assisted acid hydrolysis-high performance liquid chromatography.Methods After microwave assisted hydrolysis with HCl→MeOH(3→10),the cyanidin and peonidin in purple sweet potato were determined by HPLC.Agilent Eclipes XDB-C_(18) column(250 mm×4.6 mm,5μm)was adopted,using acetonitrile-0.1%trifluoroacetic acid solution(80:20)as mobile phase.The column temperature was set at 25℃,the flow rate was 1.0 ml/min,the detection wavelength was set at 538 nm and the injection volume was 10μl.Results The calibration curve of cyanidin was in good linearity at 0.045-0.225μg(r=0.9998)and the average recovery was 98.6%(RSD=0.88%).The calibration curve of peonidin was in good linearity at 0.102-0.510μg(r=0.9997)and the average recovery was 98.8%(RSD=1.2%).Conclusion The method is simple,accurate,reliable and reproducible,and can be used for determination of cyanidin and peonidin in purple sweet potato simultaneously.

关 键 词：紫肉甘薯 微波水解 高效液相色谱法 矢车菊素 芍药素 

分 类 号：R917[医药卫生—药物分析学]