Transcriptomic analysis of spinal cord regeneration after injury in Cynops orientalis  

作　　者：Di Wang Man Zhao Xiao Tang Man Gao Wenjing Liu Minghui Xiang Jian Ruan Jie Chen Bin Long Jun Li 

机构地区：[1]College of Life Sciences,Anhui Normal University,Wuhu,Anhui Province,China [2]College of Animal Science and Technology,Nanjing Agricultural University,Nanjing,Jiangsu Province,China [3]Laboratory of Reproductive Medicine,The Second People’s Hospital,Wuhu,Wuhu,Anhui Province,China

出　　处：《Neural Regeneration Research》2023年第12期2743-2750,共8页中国神经再生研究（英文版）

基　　金：the National Natural Science Foundation of China,Nos.32270516,31970413;the Natural Science Foundation of Anhui Province,No.1908085MC83(to JL);a Start-up grant from Nanjing Agricultural University,No.804090。

摘　　要：Cynops orientalis(C.orientalis)has a pronounced ability to regenerate its spinal cord after injury.Thus,exploring the molecular mechanism of this process could provide new approaches for promoting mammalian spinal cord regeneration.In this study,we established a model of spinal cord thoracic transection injury in C.orientalis,which is an endemic species in China.We performed RNA sequencing of the contused axolotl spinal cord at two early time points after spinal cord injury–during the very acute stage(4 days)and the subacute stage(7 days)–and identified differentially expressed genes;additionally,we performed Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses,at each time point.Transcriptome sequencing showed that 13,059 genes were differentially expressed during C.orientalis spinal cord regeneration compared with uninjured animals,among which 4273 were continuously downregulated and 1564 were continuously up-regulated.Down-regulated genes were most enriched in the Gene Ontology term“multicellular organismal process”and in the ribosome pathway at 10 days following spinal cord injury.We found that multiple genes associated with energy metabolism were down-regulated and multiple genes associated with the lysosome were up-regulated after spinal cord injury,indicating the importance of low metabolic activity during wound healing.Immune response-associated pathways were activated during the early acute phase(4 days),while the expression of extracellular matrix proteins such as glycosaminoglycan and collagen,as well as tight junction proteins,was lower at 10 days post-spinal cord injury than 4 days post-spinal cord injury.However,compared with 4 days post-injury,at 10 days post-injury neuroactive ligand-receptor interactions were no longer down-regulated,up-regulated differentially expressed genes were enriched in pathways associated with cancer and the cell cycle,and SHH,VIM,and Sox2 were prominently up-regulated.Immunofluorescence staining showed that glial fibrillary acidic protein wa

关 键 词：Cynops orientalis extracellular matrix glial fibrillary acidic protein METABOLISM NEURON RNA sequence SALAMANDER spinal cord injury spinal cord regeneration TRANSCRIPTOMICS 

分 类 号：R285.5[医药卫生—中药学]