硫酸新霉素可溶性粉中非法添加4种苯并咪唑类和3种大环内酯类抗寄生虫药物HPLC-PDA检测方法的建立  

Determination of 4 Benzimidazoles and 3 Macrolides Antiparasitic Drugs Illegally Added in Neomycin Sulfate Soluble Powder by HPLC-PDA

作　　者：王轩 张秀英[1] 赵富华[1] 熊玥 彭文绣 叶能胜[3] 韩宁宁[1] WANG Xuan;ZHANG Xiu-ying;ZHAO Fu-hua;XIONG Yue;PENG Wen-xiu;YE Neng-sheng;HAN Ning-ning(China Institute of Veterinary Drug Control,Beijing 100081,China;Jiangsu Testing Institute of Veterinary Drug and Feed,Nanjing 210036,China;Department of Chemistry,Capital Normal University,Beijing,100048,China)

机构地区：[1]中国兽医药品监察所,北京100081 [2]江苏省兽药饲料质量检验所,南京210036 [3]首都师范大学化学系,北京100048

出　　处：《中国兽药杂志》2023年第4期60-67,共8页Chinese Journal of Veterinary Drug

基　　金：北京市科学技术协会2020-2022年度青年人才托举工程项目(No.118);中国兽医药品监察所兽药行业公益性重点专项(No.GY202001、No.GY2021007)。

摘　　要：建立了硫酸新霉素可溶性粉中非法添加7种抗寄生虫药物的HPLC-PDA检测方法。采用十八烷基键合硅胶为填充剂;以水-乙腈为流动相,梯度洗脱,流速1.0 mL/min;二极管阵列检测器,波长为245 nm。通过液相色谱保留时间、峰纯度检查和光谱相似度检查对非法添加物进行确证。结果表明,该色谱条件下,7种抗寄生虫药物间分离度良好,在3~80μg/mL浓度范围内线性关系良好(R2>0.9998),回收率在103.9%~105.8%之间,RSD≤2.5%,4种苯并咪唑类和3种大环内酯类的检测限分别为0.05 g/kg和0.3 g/kg。该方法操作简便、快速、灵敏度高,可用于硫酸新霉素可溶性粉中非法添加抗寄生虫药物的测定。A method was established for the determination of seven antiparasitic drugs in neomycin soluble powder by the high-performance liquid chromatography with photo-diode detector(HPLC-PDA).C18 column was applied with mobile phase consisting of acetonitrile and water,gradient elution,at a flow rate of 1.0 mL/min.The detection wavelength was 245 nm.Retention time,peak purity test and spectrum similar test were utilized to identify the antiparasitic drugs.seven antiparasitic drugs were detected through the established method,which showed a good limits of detection(0.05 g/kg of benzimidazoles and 0.3 g/kg of macrolides)and linear range(3~80μg/mL)with a high correlation coefficients(r^(2)>0.9998).The recoveries ranged from 103.9%to 105.8%.The established method is simple,rapid and sensitive,and can be utilized for the determination of antiparasitic drugs illegally added to neomycin sulfate soluble powder.

关 键 词：硫酸新霉素可溶性粉 非法添加 抗寄生虫药物 高效液相色谱法 

分 类 号：S859.797[农业科学—临床兽医学]