胶体金免疫层析法快速检测动物源性食品中西马特罗残留  

The Gold Immunochromatography Assay for Rapid Detection of Cimaterol Residues in Animal Derived Foods

作　　者：栗瑞敏 曾望 赵小娟 岑水斌 张舒心 陈洁仪 向华[1] LI Ruimin;ZENG Wang;ZHAO Xiaojuan;CEN Shuibin;ZHANG Shuxin;CHEN Jieyi;XIANG Hua(School of Chemical Engineering and Technology,Guangdong Industry Polytechnic,Guangzhou 510300,China)

机构地区：[1]广东轻工职业技术学院轻化工技术学院,广东广州510300

出　　处：《广东轻工职业技术学院学报》2023年第2期1-6,共6页Journal of Guangdong Industry Polytechnic

基　　金：广东省自然科学基金(2018A0303130135);广州市科技基础研究计划基础与应用基础研究项目(202201011298);广东轻工职业技术学院校级人才项目(KYRC2018-016);广东省高等职业院校珠江学者岗位计划资助项目(2019)。

摘　　要：西马特罗是一种国家明令禁止被检出的“瘦肉精”类物质的一种。采用胶体金免疫层析法快速检测动物源性食品中西马特罗残留,并对西马特罗胶体金免疫层析法进行方法验证和技术评价,相对准确度98.25%,假阴性率为3.5%,假阳性率为0,满足快速检测方法验证相关标准对假阳性率和假阴性率要求。不仅建立了一种快速检测动物源性食品中西马特罗残留的胶体金免疫层析法,而且避免了含有瘦肉精的动物源性食品的流通威胁人们的健康,确保人们餐桌上的安全。Cimaterol is one of the substances known as clenbuterol,which is banned by the state.In this work,the gold immunochromatography assay was used for rapid detection of cimaterol residue in animal-derived food,and the method verification and technical evaluation of the gold immunochromatography assay of cimaterol were carried out.The relative accuracy was 98.25%,the false negative rate was 3.5%,and the false positive rate was 0,which met the requirements for false positive rate and false negative rate of the rapid detection method verification standards.Therefore,this work not only establishes a gold immunochromatography method for the rapid detection of cimaterol residues in animal-derived food,but also avoids the threat to people’s health from the circulation of animal-derived food containing clenbuterol and ensures the safety of people’s table.

关 键 词：胶体金免疫层析法 西马特罗 方法验证 动物源性食品 快速检测 

分 类 号：TS207.5[轻工技术与工程—食品科学]