不同种源米槠种子形态特征与营养成分变异分析  

Variations in seed morphological characteristics and nutritional content of Castanopsis carlesii from different provenances

作　　者：孙荣喜 潘昕昊 仲小茹 李桂盛 SUN Rongxi;PAN Xinhao;ZHONG Xiaoru;LI Guisheng(Jiangxi Provincial Key Laboratory of Silviculture,College of Forestry,Jiangxi Agricultural University,Nanchang 330045,China;Wuyuan Ecological Forest Farm,Wuyuan 333200,China)

机构地区：[1]江西省森林培育重点实验室,江西农业大学林学院,江西南昌330045 [2]婺源县生态林场,江西婺源333200

出　　处：《南京林业大学学报（自然科学版）》2023年第2期27-34,共8页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：江西省林业局科技创新专项(201801);江西省科技厅项目(20202BABL215018)。

摘　　要：【目的】揭示米槠(Castanopsis carlesii)种子形态特征与营养成分的地理变异规律,为其种质资源保护和开发利用提供科学依据。【方法】收集米槠天然分布区13个种源357个单株的种子,测定种子表型(种子长、种子宽、长宽比、体积、表面积和千粒质量)及其营养成分(可溶性糖及淀粉含量),并进行方差分析、相关分析和聚类分析。【结果】米槠种子6个表型性状在种源间和种源内均达到极显著水平(P<0.01),其中江西定南(JXDN)的种子长、宽、体积、表面积及千粒质量均最大,且与其他种源差异显著;而安徽祁门(AHQM)的千粒质量最小,仅为江西定南的66.23%,且种子长、宽、体积、表面积均较小。淀粉和可溶性糖含量(质量分数)分别为31.80%和21.76%,而且可溶性糖和淀粉含量在种源间差异达到极显著水平(P<0.01),说明营养成分含量在不同种源间相差很大,存在丰富的变异,种源间的变异系数分别为14.06%和30.62%。平均表型分化系数为28.15%,种源内变异是其表型特征遗传变异的主要来源。淀粉含量与千粒质量和种子宽呈显著正相关(P<0.05),可溶性糖含量与种子长和长宽比呈极显著负相关(P<0.01);种子长与经度呈显著负相关(P<0.05),长宽比与经度和最冷季降水量分别呈极显著负相关(P<0.01)和显著负相关(P<0.05),淀粉含量与年平均温度呈显著正相关(P<0.05)。13个种源可以划为3个类群:第Ⅰ类群包括江西定南(JXDN),属于大种子、高可溶性糖含量、高淀粉含量类群;第Ⅱ类群包括安徽祁门(AHQM)、江西信丰(JXXF)、浙江开化(ZJKH)和广东连平(GDLP),属于小种子、高可溶性糖含量、低淀粉含量类群;其他8个种源聚为第Ⅲ类群,属于中种子、中可溶性糖含量、中淀粉含量类群。地理分布相近的种源没有优先聚在一起,说明米槠种子未表现出明显的地理变异规律。【结论】米槠种子多样性是遗传和环境因素�【Objective】To provide scientific references for germplasm conservation and development and utilization of Castanopsis carlesii,the geographical variations of seed morphological characteristics and nutrient content of C.carlesii from different provenances were analyzed.【Method】Seeds of C.carlesii were collected from 357 individuals growing in natural distribution areas of thirteen provenances.Six morphological characteristics(seed length,seed width,length width ratio,volume,surface area and 1000⁃seed weight)and nutrients(soluble sugar and starch)of seeds were measured,and then variance,correlation and cluster analyses were conducted.【Result】Seeds’morphological characteristics were significantly different between and within provenances(P<0.01);the mean values of seed length,width,length width ratio,volume,surface area and 1000⁃seed weight ranged from 0.93-1.02 cm,0.82-0.93 cm,1.07-1.21,0.32-0.46 cm3,1.83-2.32 cm2 and 397.15-599.67 g,respectively.The nest analysis of variances showed that the maxima of seed length,width,volume,surface area,and 1000⁃seed weight were observed in seeds from Jiangxi Dingnan(JXDN),and significantly differed from those from other provenances.The 1000⁃seed weight of Anhui Qimen(AHQM)was 66.23%that of JXDN,and the seed length,width,volume and surface area were comparatively smaller.The percentages of starch and soluble sugar were 31.80%and 21.76%,respectively.The nutrient contents of C.carlesii were significantly different between provenances(P<0.01);the coefficients of variation were 14.06%and 30.62%.The average coefficient of phenotypic differentiation was 28.15%,and genetic variation within provenances was the main source of the genetic variation of morphology characteristics.The correlation analysis showed that starch and 1000⁃seed weight had the most positive correlations with seed width(P<0.05),while sugar had an extremely significant correlation with seed length and length⁃width ratios(P<0.01).Seed length showed a significant negative correlation with longit

关 键 词：米槠 种子 形态特征 营养成分 种源 

分 类 号：S722[农业科学—林木遗传育种] S792[农业科学—林学]