Regulation of actin cytoskeleton via photolithographic micropatterning  

作　　者：Fulin Xing Haimei Zhang Mengyu Li Hao Dong Xuehe Ma Shiyu Deng Fen Hu Imshik Lee Leiting Pan Jingjun Xu 

机构地区：[1]The Key Laboratory of Weak-Light Nonlinear Photonics of Education Ministry School of Physics and TEDA Institute of Applied Physics Nankai University,Tianjin 300071 P.R.China [2]State Key Laboratory of Medicinal Chemical Biology Frontiers Science Center for Cell Responses College of Life Sciences Nankai University,Tianjin 300071,P.R.China [3]Collaborative Innovation Center of Extreme Optics Shanxi University,Taiyuan Shanxi 030006,P.R.China [4]Shenzhen Research Institute of Nankai University Shenzhen,Guangdong 518083,P.R.China

出　　处：《Journal of Innovative Optical Health Sciences》2023年第2期50-57,共8页创新光学健康科学杂志（英文）

基　　金：This work was supported by the Guangdong Major Project of Basic and Applied Basic Research(2020B0301030009);the National Key Research and Development Program of China(2022YFC3400600)National Natural Science Foundation of China(12174208,32227802,11874231,31801134 and 31870843);Tianjin Natural Science Foundation(20JCYBJC01010);China Postdoctoral Science Foundation(2020M680032);Fundamental Research Funds for the Central Universities(2122021337 and 2122021405).

摘　　要：Actin cytoskeleton plays crucial roles in various cellular functions.Extracellular matrix(ECM)can modulate cell morphology by remodeling the internal cytoskeleton.To define how geometry of ECM regulates the organization of actin cytoskeleton,we plated individual NIH 3T3 cells on micropatterned substrates with distinct shapes and sizes.It was found that the stress fibers could form along the nonadhesive edges of T-shaped pattern,but were absent from the opening edge of V-shaped pattern,indicating that the organization of actin cytoskeleton was dependent on the mechanical environment.Furthermore,a secondary actin ring was observed on 50μm circular pattern while did not appear on 30μm and 40μm pattern,showing a size-dependent organization of actin cytoskeleton.Finally,osteoblasts,MDCK and A549 cells exhibited distinct organization of actin cytoskeleton on T-shaped pattern,suggesting a cell-type specificity in arrangement of actin cytoskeleton.Together,our findings brought novel insight into the organization of actin cytoskeleton on micropatterned environments.

关 键 词：Actin cytoskeleton PHOTOLITHOGRAPHY MICROPATTERNING extracellular matrix 

分 类 号：R318[医药卫生—生物医学工程]