海藻酸钠-多聚赖氨酸复合益生菌微胶囊的构建及性能评价  

Construction and Performance Evaluation of Sodium Alginate Poly-L-Lysine Compound Probiotics Microcapsule

作　　者：张琳[1,2] 尚校兰[2] 李秋玲 齐颖 ZHANG Lin;SHANG Xiaolan;LI Qiuling;QI Ying(Langfang Normal University,Hebei Key Laboratory of Animal Diversity,Langfang 065000,Hebei China;Langfang Normal University,Langfang Key Laboratory of Food Nutrition and Safety,Langfang 065000,Hebei China)

机构地区：[1]廊坊师范学院,河北省动物多样性重点实验室,河北廊坊065000 [2]廊坊师范学院,廊坊市食品营养与安全重点实验室,河北廊坊065000

出　　处：《饲料博览》2023年第2期30-35,共6页Feed Review

基　　金：河北省自然科学基金项目(C2020408003);廊坊市科学技术研究与发展计划自筹经费项目(2020013020)。

摘　　要：文章旨在研究海藻酸钠-多聚赖氨酸复合益生菌微胶囊的抗胁迫能力。试验以海藻酸钠和多聚赖氨酸为壁材,采用内源乳化法微囊化益生菌。观察微胶囊益生菌的形态,测定微胶囊颗粒的粒径,并对益生菌微胶囊的耐温、耐酸、肠溶性等特性展开研究,以未包被的益生菌菌粉为对照,在高温110℃和130℃处理30、45 s和60 s;在100%高湿度下于75℃和85℃的条件下处理1 min;在模拟胃液和肠液中处理30、90 min和180 min。结果显示:益生菌微胶囊大小均一,平均粒径是717μm;与未包被的益生菌菌粉相比,益生菌微胶囊抵抗高温、高湿的能力极显著提升(P<0.01);益生菌微胶囊耐胃液、肠液的能力显著提高(P<0.05)。结果说明海藻酸钠-多聚赖氨酸复合益生菌微胶囊的抗胁迫能力要显著高于未包被菌粉。The stress resistance of sodium alginate and poly-l-lysine compound probiotics microcapsules was studied.Sodium alginate and poly-l-lysine were used as wall materials,and the probiotics were pre-fermented encapsulated by the internal emulsification method.The morphological structures of microcapsule probiotics and the diameter distribution of microcapsule granule were observed and measured.The characteristics of temperature resistance,acid resistance and intestinal solubility of probiotics microcapsules were studied.Compared with unencapsulated probiotics bacterial powder,the microencapsulated bacteria were treated at 110 or 130℃ for 30,45 s and 60 s,respectively;and were treated at 75 or 85℃ for 1 min under the 100% high humidity treatment;and were treated in simulated gastric or intestinal fluids for 30,90 min and 180 min treatment.The results showed probiotic microcapsules were uniform in size,and the average diameter of the microcapsules was 717μm.Compared with unencapsulated probiotics bacterial powder,the ability to resist high temperature and high humidity was improved significantly(P<0.01),and the ability to tolerate gastric juice and intestinal juice of probiotics microcapsules was significantly improved(P<0.05).The stress resistance of sodium alginate and poly-l-lysine compound probiotics microcapsule was much higher than that of uncapsulated ones.

关 键 词：益生菌 微胶囊 抗胁迫 粪肠球菌 

分 类 号：S816.3[农业科学—饲料科学]