喉鳞癌EBV感染危险因素及其对TCAB1、c-myc基因表达的影响  

Risk factors for EBV infection in patients with laryngeal squamous cell carcinoma and influence on expressions of TCAB1 and c-myc gene

作　　者：刘勇 晁方 秦刚 牟丽莉 厉波 LIU Yong;CHAO Fang;QIN Gang;MOU Li-li;LI Bo(Rizhao Central Hospital,Rizhao,Shandong 276800,China;不详)

机构地区：[1]日照市中心医院耳鼻喉科,山东日照276800 [2]日照市人民医院麻醉手术一部,山东日照276800 [3]日照市中心医院口腔科,山东日照276800 [4]日照市中心医院药学部,山东日照276800 [5]日照市中心医院泌尿外科,山东日照276800

出　　处：《中华医院感染学杂志》2023年第5期757-761,共5页Chinese Journal of Nosocomiology

基　　金：山东省科研基金资助项目(2019H1201)。

摘　　要：目的分析喉鳞癌爱泼斯坦-巴尔病毒(EBV)感染的危险因素,并评估其对端粒酶卡扎尔体蛋白1(TCAB1)、c-myc基因表达的影响。方法回顾性分析2016年1月-2021年8月日照市中心医院收治的92例行手术治疗的喉鳞癌患者临床资料,根据患者癌组织及癌旁组织EBV感染情况,分为EBV阳性组55例和EBV阴性组37例,比较两组临床特征差异,分析影响喉鳞癌患者EBV感染的危险因素;并比较EBV阳性组与EBV阴性组癌组织TCAB1及c-myc基因表达水平及外周血EBV-DNA水平,评估喉鳞癌患者外周血EBV-DNA水平与TCAB1及c-myc基因表达水平的相关性。结果两组性别、年龄、肿瘤部位、临床分期、分化程度及吸烟史比较,无统计学差异,但EBV阳性组淋巴结转移率高于EBV阴性组(P<0.05),Logistic回归分析显示,淋巴结转移是影响喉鳞癌患者EBV感染的危险因素(P<0.05)。EBV阳性组癌组织TCAB1及c-myc mRNA相对表达量、外周血EBV-DNA水平均高于EBV阴性组(P<0.05),Pearson相关性分析显示,喉鳞癌患者外周血EBV-DNA水平与癌组织TCAB1及c-myc mRNA相对表达量呈正相关(P<0.05)。结论EBV感染可能参与喉鳞癌的发生发展,其作用机制可能与上调TCAB1、c-myc的表达有关。OBJECTIVE To analyze the risk factors for Epstein-Barr virus(EBV)infection in patients with laryngeal squamous cell carcinoma and observe the influence on expressions of telomerase cajal body protein 1(TCAB1)and c-myc gene.METHODS Totally 92 patients with laryngeal squamous cell carcinoma who received surgical procedures in Rizhao Central Hospital from Jan 2016 to Aug 2021 were enrolled in the study and divided into the EBV-positive group with 55 cases and the EBV-negative group with 37 cases according to the status of EBV infection in cancer tissues and adjacent tissues,and the clinical data of the patients were retrospectively analyzed.The clinical characteristics were compared between the two groups,the risk factors for EBV infection in the patients with laryngeal squamous cell carcinoma were analyzed.The expression levels of TCAB1 and c-myc gene in cancer tissues and peripheral blood EBV-DNA level were compared between the EBV-positive group and the EBV-negative group.The association between the peripheral blood EBV-DNA level and the expression levels of TCAB1 and c-myc gene was observed.RESULTS There were no significant differences in the genders,age,tumor sites,clinical stage,degree of differentiation and smoking history between the two groups,the lymphatic metastasis rate of the EBV-positive group was higher than that of the EBV-negative group(P<0.05).Logistic regression analysis showed that the lymphatic metastasis is the risk factor for EBV infection in the patients with laryngeal squamous cell carcinoma(P<0.05).The relative expression levels of cancer tissue TCAB1 and c-myc mRNA and peripheral blood EBV-DNA level were higher in the EBV-positive group than in the EBV-negative group(P<0.05).Pearson correlation analysis indicated that the peripheral blood EBV-DNA level of the patients with laryngeal squamous cell carcinoma was positively correlated with the relative expression levels of TCAB1 and c-myc mRNA in cancer tissues(P<0.05).CONCLUSION The EBV infection may be involved in the pathogenesis of laryng

关 键 词：喉鳞癌 爱泼斯坦-巴尔病毒 感染 危险因素 端粒酶卡扎尔体蛋白1 C-MYC 端粒酶 

分 类 号：R739.65[医药卫生—肿瘤]