1株牛病毒性腹泻病毒的分离鉴定及其结构蛋白E2序列分析  

Isolation,Identification and Structural Protein E2 Sequence Analysis of Bovine Viral Diarrhea Virus

作　　者：王妍瑾 宋涛 杨雨杰 金宇航 乔天一 孟赓 严世杰 芮萍[1] 马增军[1] WANG Yan-jin;SONG Tao;YANG Yu-jie;JIN Yu-hang;QIAO Tian-yi;MENG Geng;YAN Shi-jie;RUI Ping;MA Zeng-jun(College of Animal Science and Technology,Hebei Normal University of Science and Technology,Qinhuangdao 066600,China;College of Veterinary Medicine,China Agricultural University,Beijing 100193,China)

机构地区：[1]河北科技师范学院动物科技学院,河北秦皇岛066600 [2]中国农业大学动物医学院,北京海淀100193

出　　处：《中国兽医杂志》2023年第4期13-18,共6页Chinese Journal of Veterinary Medicine

基　　金：河北省研究生创新资助项目(CXZZSS2020119);河北省奶业振兴重大技术创新专项(19226631D);秦皇岛市科技计划项目(201903B009)。

摘　　要：为了解河北省唐山市规模化奶牛场中牛病毒性腹泻病毒(BVDV)流行毒株的基因特征,本试验于唐山市2个规模化奶牛场采集了49份临床样品进行逆转录聚合酶链式反应(RT-PCR)检测,并对阳性样品进行病毒的分离,最终成功分离到1株非致细胞病变的BVDV毒株,命名为HB-1株。电镜观察结果显示,HB-1株具有典型的BVDV病毒粒子形态。对HB-1株全基因组序列进行扩增和测序,基因遗传进化分析结果显示,HB-1株属于BVDV-1m亚型。HB-1株结构蛋白E2全长序列糖基化位点和抗原表位预测结果显示,HB-1株E2蛋白含有4个保守的糖基化位点,其中抗原表位1高度保守。本试验为河北省进一步开展牛病毒性腹泻(BVD)流行病学调查、疫苗株筛选以及检测方法建立提供数据支撑。In order to assess the genetic characteristics of bovine viral diarrhea virus(BVDV)epidemic strains in large-scale dairy farms in Tangshan,Hebei province,this study collected 49 clinical samples from two large-scale dairy farms in Tangshan for reverse transcription-polymerase chain reaction(RT-PCR)detection,leading to the isolation of a non-cytopathic BVDV strain,named HB-1.The morphology of BVDV virus particles was observed by electron microscope,the whole genome sequence of HB-1 strain was amplified and analyzed,and the glycosylation and antigenic epitopes of HB-1 strain E2 protein sequence were predicted.The electron microscope results revealed that HB-1 strain had typical morphology of BVDV;the whole genome sequence analysis showed that HB-1 strain belonged to the subtype of BVDV-1m;the E2 protein sequence analysis indicated that HB-1 strain E2 protein contained four conserved glycosylation sites,and its epitope 1 was highly conserved.These results provide data support for the further epidemiological investigation of BVD,the screening of vaccine strains and the establishment of detection methods in Hebei province.

关 键 词：牛病毒性腹泻病毒 分离和鉴定 全基因组测序 E2蛋白序列分析 

分 类 号：S854.4[农业科学—临床兽医学]