基于超高效液相色谱-四级杆-飞行时间质谱技术分析清利湿热颗粒化学成分  

Chemical Component Analysis of Qingli Shire Granules Based on Ultra-performance Liquid Chromatography Quadrupole Time-of-flight Mass Spectrometry

作　　者：南海鹏 刘金莲 陈亚文 陶然 邸松蕊 张建军 王淳 陈洪璋 李波[4] 王林元[1] NAN Haipeng;LIU Jinlian;CHEN Yawen;TAO Ran;DI Songrui;ZHANG Jianjun;WANG Chun;CHEN Hongzhang;LI Bo;WANG Linyuan(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 100029,China;School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China;Infinitus(China)Co.,LTD.,Guangzhou 529156,China;National Institutes for Food and Drug Control,Beijing 102629,China)

机构地区：[1]北京中医药大学中药学院,北京100029 [2]北京中医药大学中医学院,北京100029 [3]无限极(中国)有限公司,广州529156 [4]中国食品药品检定研究院,北京102629

出　　处：《世界中医药》2023年第6期748-755,共8页World Chinese Medicine

基　　金：国家重点研发计划项目(2018YFC1706800)。

摘　　要：目的:采用超高效液相色谱-四级杆-飞行时间质谱(UPLC-Q-TOF-MS)技术初步鉴定经水提、浓缩、干燥、制粒后的清利湿热颗粒中的化学成分并确定其药味归属。方法:采用Agilent ZORBAX RRHD Eclipse XDB-C18色谱柱(2.1 mm×100 mm,1.8μm),以0.1%甲酸水溶液-乙腈为流动相体系,梯度洗脱,进样量为5μL,在正、负离子模式下对处理后的清利湿热颗粒进行质谱检测。结果:通过与Natural Products HR-MS/MS Spectral Library 1.0数据库对比、文献分析以及质谱裂解规律从清利湿热颗粒样品中鉴定出54个化合物。结论:本研究为清利湿热颗粒质量控制及确定该产品可能的药效物质基础提供了依据。Objective:To identify the chemical components of Qingli Shire Granules after water extraction,concentration,drying and granulation and determine the attribution of their medicinal flavor by ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS).Methods:The determination was performed on an Agilent ZORBAX RRHD Eclipse XDB-C18 column(2.1 mm×100 mm,1.8μm)with gradient elution of 0.1%formic acid aqueous solution and acetonitrile as mobile phase system.The sample size was 5μL.The treated Qingli Shire Granules were detected by mass spectrometry under positive and negative ion modes.Results:Fifty-four compounds were identified from the samples by comparing with Natural Products HR-MS/MS Spectral Library 1.0 database,literature analysis,and cracking rules of mass spectrometry.Conclusion:This study provides a basis for the quality control of Qingli Shire Granules and preliminarily determines the possible pharmacodynamic substance basis of this product.

关 键 词：清利湿热颗粒 化学成分 超高效液相色谱-四级杆-飞行时间质谱 京尼平苷酸 毛蕊花糖苷 杯苋甾酮 药效物质 

分 类 号：R286.0[医药卫生—中药学]