食品及临床样本中沙门菌多重耐药相关基因的研究  

Study on multidrug resistance related genes of Salmonella isolated from food and clinical samples

作　　者：张铭琰 耿英芝[1] 于淼[1] 张眉眉[1] ZHANG Mingyan;GENG Yingzhi;YU Miao;ZHANG Meimei(Liaoning Provincial Center for Disease Control and Prevention,Liaoning Shenyang 110005,China)

机构地区：[1]辽宁省疾病预防控制中心,辽宁沈阳110005

出　　处：《中国食品卫生杂志》2023年第2期184-189,共6页Chinese Journal of Food Hygiene

摘　　要：目的了解从食品及临床样本中分离的沙门菌整合子以及产超广谱β-内酰胺酶(ESBL)菌分布,以及不同耐药基因与多重耐药之间的关系。方法利用K-B法进行产ESBL菌表型确证试验;通过聚合酶链式反应方法,对产超广谱β-内酰胺酶沙门菌相关耐药基因(bla_(TEM)、bla_(SHV)、bla_(CTX))以及可移动元件—整合子基因进行扩增,对整合子可变区扩增产物进行基因测序,分析携带耐药基因盒。结果共分离309株沙门菌,138株来自食品(禽类96株,生猪肉19株,水产品23株),171株来自临床样本。309株沙门菌耐药率达78.3%,多重耐药率达41.1%,其中禽类耐药率占比最高。共检出56株产ESBL菌,其中35株携带产ESBL菌相关耐药基因(bla_(TEM)基因型15株,bla_(CTX)基因型10株,bla_(TEM)与bla_(CTX)双基因型10株),未检出bla_(SHV)基因。检出98株Ⅰ类整合子阳性菌,阳性率为31.7%。其中54株携带耐药基因盒,47株携带dfrA以及aadA基因,1株携带linG-aadA22基因盒,其余为空基因盒。整合子阳性菌和产ESBL菌多重耐药率分别高达98.0%和89.3%。产ESBL菌中整合子阳性率高达76.8%(43/56)。结论本地区产ESBL与Ⅰ类整合子沙门菌分布广泛,产ESBL菌基因型以bla_(TEM)和bla_(CTX)为主。整合子与产ESBL菌均与多重耐药有关。该地区沙门菌耐药形势严峻,禽类养殖中尤需注意对抗生素的规范使用。Objective To investigate distribution of integron and extended-spectrumβ-lactamase produced by Salmonella isolated from food and clinical samples,and explore the relationship between different drug resistance genes and multidrug resistance.Methods Phenotype of ESBL-producing strains were confirmed by K-B method.The ESBL related drug resistance genes(bla_(TEM),blaSHV,bla_(CTX))and the mobile element integron in Salmonella were amplified by polymerase chain reaction.The amplified products of integron variable region were sequenced and the drug resistance gene cassettes were analyzed.Results Three hundred and nine Salmonella strains were isolated.A total of 138 Salmonella strains were isolated from food,including poultry(n=96),raw pork(n=19)and aquatic products(n=23).One hundred and seventy one Salmonella strains were isolated from clinical samples.The drug resistance rate of 309 Salmonella strains was 78.3%,and the multidrug resistance rate was 41.1%.The antimicrobial resistance rate of poultry was the highest.A total of 56 ESBLproducing strains were detected,of which 35 strains carried the ESBL genes(15 strains carried bla_(TEM);10 strains carried bla_(CTX),10 strains carried bla_(TEM)and bla_(CTX)).The blaSHV gene was not detected.A total of 98 strains which carried class I integron gene were detected,and the positive rate was 31.7%.Among the 98 strains,54 strains carried drug resistance gene cassette.Forty seven strains carried dfrA and aadA,1 strain carried linG-aadA22,the rest were empty gene cassette.The multi-drug resistance rates of integron positive and ESBL-producing strains were as high as 98.0%and 89.3%,respectively.The positive rate of integron in ESBL-producing strains was 76.8%(43/56).Conclusion The class I integron and ESBLproducing Salmonella were widely distributed in this area.The genotypes of ESBL-producing strains were mainly bla_(TEM)and bla_(CTX).Both integron and ESBL-producing strains were associated with multidrug resistance.The dug resistance situation of Salmonella in this area was seri

关 键 词：沙门菌 耐药性 整合子 ESBL 

分 类 号：R155[医药卫生—营养与食品卫生学]