茶树NUDIX基因家族的鉴定及表达分析  

Identification and Expression Pattern Analysis of NUDIX Gene Family in Camellia sinensis

作　　者：陈振艳 张相琴 陈兰 谢思艺 刘硕谦[1,2] 田娜[1,2] CHEN Zhenyan;ZHANG Xiangqin;CHEN Lan;XIE Siyi;LIU Shuoqian;TIAN Na(Key Laboratory of Tea Science of Ministry of Education,Changsha 410128,China;Department of tea science,College of horticulture,Hunan Agricultural university,Changsha 410128,China)

机构地区：[1]湖南农业大学园艺学院茶学教育部重点实验室,湖南长沙410128 [2]湖南农业大学园艺学院茶学系,湖南长沙410128

出　　处：《茶叶科学》2023年第2期159-172,共14页Journal of Tea Science

基　　金：国家自然科学基金(32172629);湖南省种业创新项目(2021NK1008)。

摘　　要：NUDIX水解酶属于焦磷酸酶,在信息传递、植物生长协调和响应逆境胁迫等方面起着重要作用。基于茶树(Camellia sinensis)基因组鉴定出43个NUDIX家族基因,并运用生物信息学和实时荧光定量PCR等方法对其分析。结果表明,43个CsNUDXs蛋白质分子量介于11.8~89.2 kDa,氨基酸长度为102~342个,理论等电点为4.49~9.26,18.6%的蛋白为稳定性蛋白;根据进化关系将CsNUDXs分为6个亚家族;启动子顺式作用元件分析发现CsNUDXs具有许多与激素响应、逆境胁迫和生长发育等调控相关的作用元件;对CsNUDXs家族基因在不同器官表达模式进行分析,发现CsNUDX3、CsNUDX7在果实中表达量较高,而CsNUDX22、CsNUDX25在果实中表达量极低,CsNUDX30在老叶中表达量极低;不同处理组的实时荧光定量PCR结果表明,1 mmol·L^(-1)茉莉酸甲酯(MeJA)处理下CsNUDX1、CsNUDX2和CsNUDX33等表达量呈现先升后降再升的趋势,而1 mmol·L^(-1)水杨酸(SA)处理下CsNUDX4、CsNUDX12和CsNUDX22等表达量则呈现先降后升再降的趋势,300 mmol·L^(-1)NaCl处理下CsNUDX2、CsNUDX4和CsNUDX22等表达量呈现先升后降的趋势。本研究应用生物信息学技术初步解析了CsNUDXs的基本特征和功能,发现CsNUDXs可响应高盐胁迫和MeJA、SA处理。NUDIX hydrolase belongs to pyrophosphatase,which plays an important role in information transmission,plant growth coordination and responses to adversity stresses.In this study,43 NUDIX family genes were identified based on the tea(Camellia sinensis)genome,and analyzed by bioinformatics and fluorescence quantitative PCR.The results show that the protein molecular weight of 43 CsNUDXs rang from 11.8~89.2 kDa,with 102 to 342 amino acids.The theoretical isoelectric point was from 4.49 to 9.26,and 18.6%of them were stable proteins.According to the evolutionary relationship,CsNUDXs is divided into six subfamilies.Cis-acting element analysis of promoter shows that CsNUDXs have many functional elements related to hormone response,adversity stress,growth and development.The expression patterns of CsNUDXs in different organs were analyzed.It was found that the expression levels of CsNUDX3 and CsNUDX7 were high in fruits,while the expression levels of CsNUDX22 and CsNUDX25 were extremely low in fruits,and the expression level of CsNUDX30 was extremely low in old leaves.In addition,the results of real-time fluorescence quantitative PCR show that the expression levels of CsNUDXs,such as CsNUDX1,CsNUDX2 and CsNUDX33 increased first,then decreased and then increased under the treatment of 1 mmol·L^(-1) MeJA.However,under the treatment of 1 mmol·L^(-1) SA,the expression levels of CsNUDX4,CsNUDX12 and CsNUDX22 decreased first,then increased and then decreased.While under the treatment of 300 mmol·L^(-1) NaCl,the expression levels of CsNUDX2,CsNUDX4 and CsNUDX22 increased first and then decreased.In summary,the basic characteristics and functions of CsNUDXs were preliminarily analyzed by bioinformatics technology,and it was found that CsNUDXs could respond to high salt stress,MeJA and SA treatments.

关 键 词：茶树 NUDIX基因家族 非生物胁迫 表达分析 

分 类 号：S571.1[农业科学—茶叶生产加工] Q786[农业科学—作物学]