免疫组化法与RNAscope原位杂交法检测宫颈鳞状细胞癌组织中PD⁃1和PD⁃L1表达一致性研究  

Study on correlation between the expression consistency of PD⁃1 and PD⁃L1 in cervical squamous cell carcinoma tissues by immunohistochemical method and in RNAscopy situ hybridization

作　　者：鲍书友 李丽[1] 劳吉锋[1] 倪建 Bao Shuyou;Li Li;Lao Jifeng;Ni Jian(Department of Pathology,Jiangyin Traditional Chinese Medicine Hospital,Wuxi 214400,China)

机构地区：[1]江阴市中医院病理科,江苏无锡214400 [2]徐州市中心医院病理科

出　　处：《海军医学杂志》2023年第3期305-309,共5页Journal of Navy Medicine

摘　　要：目的研究免疫组化法与RNAscope原位杂交法检测宫颈鳞状细胞癌组织中程序性死亡分子1(PD⁃1)和程序性死亡分子1配体(PD⁃L1)表达一致性。方法回顾性分析2019年3月至2021年2月被江阴市中医院病理科诊断为宫颈鳞状细胞癌的80例患者石蜡组织标本。采用免疫组化法与RNAscope原位杂交法测定宫颈鳞状细胞癌组织中PD⁃1和PD⁃L1表达情况,分析此2种方法检测癌组织中PD⁃1和PD⁃L1表达一致性。分析RNAscope原位杂交法检测癌组织中PD⁃1mRNA、PD⁃L1mRNA表达与其临床病理特征的关系。随访1年,记录所有患者总生存时间。结果免疫组化法结果显示,80例宫颈鳞状细胞癌组织标本中PD⁃1蛋白阳性52例,阳性率为65.00%;PD⁃L1蛋白阳性60例,阳性率为75.00%。RNAscope原位杂交法结果显示,80例宫颈鳞状细胞癌组织标本中PD⁃1蛋白阳性49例,阳性率为61.25%;PD⁃L1蛋白阳性57例,阳性率为71.25%。免疫组化法与RNAscope原位杂交法检测PD⁃1表达一致性良好(Kappa值=0.847,P>0.05);免疫组化法与RNA⁃scope原位杂交法检测PD⁃L1表达一致性良好(Kappa值=0.861,P>0.05)。PD⁃1mRNA、PD⁃L1mRNA表达与患者年龄、脉管侵犯、淋巴结转移、神经侵犯、肿瘤分期(TNM)、肿瘤直径无关(P>0.05)。随访1年,PD⁃1mRNA阳性表达与PD⁃1mRNA阴性表达的总生存曲线比较差异无统计学意义(P>0.05);PD⁃L1mRNA阳性表达与PD⁃L1mRNA阴性表达的总生存曲线比较差异无统计学意义(P>0.05)。结论免疫组化法与RNAscope原位杂交法检测宫颈鳞状细胞癌组织中PD⁃1和PD⁃L1表达一致性较好,且PD⁃1mRNA、PD⁃L1mRNA表达与患者总生存时间无关。Objective To study the expression consistency of programmed death molecule 1(PD⁃1)and programmed death molecule 1 ligand(PD⁃L1)in cervical squamous cell carcinoma tissues by immunohistochemical method and in RNAscopy situ hybridization.Methods Paraffin tissue samples of 80 cases diagnosed as cervical squamous cell carcinoma in the Pathology Department of Jiangyin Traditional Chinese Medicine Hospital from March 2019 to February 2021 were retrospectively analyzed.The expression levels of PD⁃1 and PD⁃L1 in cervical squamous cell carcinoma tissues were determined by immunohistochemical method and RNAscope in situ hybridization.The consistency of the expression of PD⁃1 and PD⁃L1 in the cancer tissues was analyzed by the two methods,and the relationship between the expressions of PD⁃1 mRNA and PD⁃L1mRNA in cancer tissues and their clinicopathological features was analyzed by RNAscope in situ hybridization.The patients had medical follow⁃ups for 1 year and the overall survival time of all the patients was recorded.Results Immunohistochemical assay showed that in 80 cases of cervical squamous cell carcinoma,52 cases were PD⁃1 protein positive,accounting for 65.00%and 60 cases were PD⁃L1 protein positive,accounting for 75.00%.The results of in situ hybridization by RNAscope showed that 49 cases were PD⁃1 protein positive,accounting for 61.25%and 57 cases were PD⁃L1 positive,accounting for 71.25%,in 80 cases of cervical squamous cell carcinoma.There was good consistency by immunohistochemistry and RNAscope in situ hybridization(Kappa=0.847)(P>0.05).There was also good consistency by immunohistochemistry and RNAscope in situ hybridization(Kappa=0.861)(P>0.05).The expression of PD⁃1 mRNA and PD⁃L1 mRNA were not correlated with age,vascular invasion,lymph node metastasis,nerve invasion,tumor node metastasis(TNM)stage and tumor diameter(P>0.05).One⁃year follow⁃up revealed that there was no statistical significance in the overall survival curve,when comparisons were made between PD⁃1 mRNA po

关 键 词：免疫组化法 程序性死亡分子1 RNAscope原位杂交法 宫颈鳞状细胞癌 表达一致性 程序性死亡分子1配体 

分 类 号：R737.3[医药卫生—肿瘤]