莪术醇通过调节lncRNA NR2F1-AS1/miR-145-5p表达抑制前列腺癌细胞增殖及转移  

Curcumol inhibits proliferation and metastasis of prostate cancer cells by regulating expression of lncRNA NR2F1-AS1/miR-145-5p

作　　者：汪洋[1] 杨君[1] WANG Yang;YANG Jun(Department of Urology,Xianning Central Hospital,the First Affiliated Hospital of Hubei University of Science and Technology,Xianning 437100,China)

机构地区：[1]咸宁市中心医院,湖北科技学院附属第一医院泌尿外科,咸宁437100

出　　处：《中国免疫学杂志》2023年第4期745-749,共5页Chinese Journal of Immunology

摘　　要：目的:探讨莪术醇对前列腺癌细胞增殖及转移的影响及其可能作用机制。方法:不同浓度的莪术醇处理人前列腺癌细胞LNCap,采用脂质体转染法将si-NC、si-lncRNA NR2F1-AS1分别转染至LNCap细胞,将pcDNA、pcDNA-lncRNA NR2F1-AS1分别转染至LNCap细胞后加入100μg/ml莪术醇处理;CCK-8法、平板克隆形成实验、Transwell实验分别检测细胞增殖、克隆形成、迁移及侵袭;qRT-PCR检测lncRNA NR2F1-AS1、miR-145-5p表达量;双荧光素酶报告实验验证lncRNA NR2F1-AS1与miR-145-5p的靶向关系。结果:莪术醇可降低细胞存活率,并可降低lncRNA NR2F1-AS1表达量,克隆形成数、迁移及侵袭细胞数减少(P<0.05),而miR-145-5p表达量升高(P<0.05);lncRNA NR2F1-AS1可靶向调控miR-145-5p的表达(P<0.05);转染si-lncRNA NR2F1-AS1后细胞存活率降低(P<0.05),miR-145-5p表达量升高(P<0.05),克隆形成数、迁移及侵袭细胞数减少(P<0.05);转染pcDNA-lncRNA NR2F1-AS1可降低莪术醇对LNCap细胞增殖及转移的作用。结论:莪术醇可通过调节lncRNA NR2F1-AS1/miR-145-5p表达,抑制前列腺癌细胞增殖、克隆形成、迁移及侵袭。Objective:To explore the effect of curcumol on the proliferation and metastasis of prostate cancer cells and its pos‐sible mechanism.Methods:Different concentrations of curcumol were used to treat human prostate cancer cells LNCap.si-NC,si-lnc-RNA NR2F1-AS1,pcDNA and pcDNA-lncRNA NR2F1-AS1 were transfected into LNCap cells by liposome transfection method re‐spectively.CCK-8 method,plate colony formation experiment,Transwell experiment were used to detect cell proliferation,colony for‐mation,migration and invasion.qRT-PCR was used to detect the expression of lncRNA NR2F1-AS1 and miR-145-5p.The dual lucifer‐ase report experiment was used to verify the targeting relationship between lncRNA NR2F1-AS1 and miR-145-5p.Results:Curcurmol decreased the cell survival rate and the expression of lncRNA NR2F1-AS1,and the number of colony formation,migration and inva‐sion cells were decreased(P<0.05),while the expression of miR-145-5p was increased(P<0.05).lncRNA NR2F1-AS1 could target the expression of miR-145-5p(P<0.05),while the cell survival rate was decreased(P<0.05),and the numbers of clone formation,migration and invasion cells were decreased(P<0.05).pcDNA-lncRNA NR2F1-AS1 transfected could reduce the effect of curcumol on the proliferation and metastasis of LNCap cells.Conclusion:Curcumol can inhibit the proliferation,clone formation,migration and invasion of prostate cancer cells by regulating the expression of lncRNA NR2F1-AS1/miR-145-5p.

关 键 词：莪术醇 前列腺癌 lncRNA NR2F1-AS1 miR-145-5p 细胞增殖 迁移 侵袭 

分 类 号：R737.25[医药卫生—肿瘤]