miR-551a拮抗蟾毒灵对肝癌细胞增殖和转移的效应  

miR-551a antagonizes effect of bufalin on proliferation and metastasis of liver cancer cells

作　　者：寇卫政[1] 赵红珂 王方 王亚楠 黄海波[1] 寇小格[1] KOU Weizheng;ZHAO Hongke;WANG Fang;WANG Yanan;HUANG Haibo;KOU Xiaoge(The Third Ward of Oncology Department,the First Affiliated Hospital of Xinxiang Medical College,Weihui 453100,China)

机构地区：[1]新乡医学院第一附属医院肿瘤科三病区,卫辉453100

出　　处：《中国免疫学杂志》2023年第4期798-803,共6页Chinese Journal of Immunology

摘　　要：目的:研究蟾毒灵对肝癌细胞增殖和转移影响的分子机制。方法:人肝癌细胞SK-Hep1分为NC组、蟾毒灵低、高剂量组、anti-miR-NC组、anti-miR-551a组、miR-NC组、miR-551a组、高剂量组+miR-NC组、高剂量组+miR-551a组。四甲基偶氮唑盐比色法(MTT)检测细胞增殖活性;流式细胞仪检测细胞凋亡;Transwell检测细胞迁移和侵袭;实时荧光定量PCR(RT-qPCR)检测miR-551a表达水平。结果:蟾毒灵处理的SK-Hep1细胞活性降低,细胞凋亡率升高,细胞迁移、侵袭数量减少,miR-551a表达水平降低(P<0.05)。抑制miR-551a表达后,SK-Hep1细胞活性降低,细胞凋亡率升高,细胞迁移、侵袭数量减少(P<0.05)。过表达miR-551a逆转了蟾毒灵对肝癌细胞的影响。结论:蟾毒灵可能通过抑制miR-551a的表达抑制肝癌细胞的增殖和转移,促进细胞凋亡。Objective:To investigate the molecular mechanism of bufalin affecting the proliferation and metastasis of liver can‐cer cells.Methods:Human liver cancer cell SK-Hep1 were divided into NC group,bufalin low and high dose group,anti-miR-NC group,anti-miR-551a group,miR-NC group,miR-551a group,high-dose group+miR-NC group,high-dose group+miR-551a group.MTT was used to detect cell proliferation activity;flow cytometry to detect cell apoptosis;Transwell was used to detect cell migration and invasion;RT-qPCR was used to detect the expression level of miR-551a.Results:Bufalin-treated SK-Hep1 cell activity was de‐creased,the rate of apoptosis was increased,the number of cell migration and invasion were decreased,and the expression level of miR-551a was decreased(P<0.05).After inhibiting the expression of miR-551a,SK-Hep1 cell activity was decreased,cell apoptosis rate was increased,and the number of cell migration and invasion were decreased(P<0.05).Overexpression of miR-551a reversed the effect of bufalin on liver cancer cells.Conclusion:Bufalin may inhibit the proliferation and metastasis of liver cancer cells,and pro‐mote cell apoptosis by inhibiting the expression of miR-551a.

关 键 词：蟾毒灵 miR-551a 肝癌 增殖 转移 

分 类 号：R735.7[医药卫生—肿瘤]