用于活细胞持续动态观测的原位培养系统设计  

Design of an in-situ Culture System for Continuous Dynamic Observation of Living Cells

作　　者：吴彤 李昱龙 林恺铖 葛明锋 董文飞 WU Tong;LI Yu-long;LIN Kai-cheng;GE Ming-feng;DONG Wen-fei(School of Mechanical and Electrical Engineering,Changchun University of Science and Technology,Changchun 130013,China;Suzhou Institute of Biomedical Engineering and Technology,Chinese Academy of Sciences,Suzhou 215163,China;Suzhou Guoke Medical Technology Development(Group)Co.,Ltd.,Suzhou 215163,China)

机构地区：[1]长春理工大学机电工程学院,长春130013 [2]中国科学院苏州生物医学工程技术研究所,苏州215163 [3]苏州国科医工科技发展(集团)有限公司,苏州215163

出　　处：《科学技术与工程》2023年第10期4100-4110,共11页Science Technology and Engineering

基　　金：中科院仪器设备研制项目(YJKYYQ20200038);江苏省重点研发计划(BE2019683)。

摘　　要：为满足对活细胞进行数小时以上跟踪观测的实验需求,设计了一种能放置在显微镜载物台上对其进行持续动态观测的原位恒温培养系统。系统以微控制器STM32为核心,以增量式PID算法实现对温度的高精度控制,采用引入权重矩阵的分布式温度控制技术保证了微型培养箱内的温度高均匀态。实验结果表明,系统温度控制精度为±0.10℃,温度均匀性为±0.20℃。通过在系统中连续培养金黄色葡萄球菌3 h,对比标准37℃恒温培养箱始终状态下细菌菌群平均面积增长率间无显著性差异(P>0.05),证明了系统性能满足实验所需,同时,对比在标准培养箱下同步进行的间断细菌培养对照组细菌生长状态,证明了生长环境温度的改变对细菌活性有负面影响,原位培养系统能够提高实验效率和实验结果可靠性。In order to meet the experimental requirements of tracking and observing living cells for more than several hours,an in-situ constant temperature culture system was designed that can be placed on the microscope stage for continuous dynamic observation.The system takes the microcontroller STM32 as the core,uses the incremental PID algorithm to achieve high-precision temperature control,and adopts the distributed temperature control technology that introduces the weight matrix to ensure the high uniformity of temperature in the micro incubator.The experimental results show that the temperature control accuracy of the system is±0.10℃,and the temperature uniformity is±0.20℃.By continuously culturing Staphylococcus aureus in the system for 3 hours,there was no significant difference(P>0.05)in the average area growth rate of bacterial flora compared with the standard 37°C constant temperature incubator,which proves that the performance of the system meets the experimental requirements.At the same time,compared with the bacterial growth state of the control group of intermittent bacterial culture synchronously in a standard incubator,it is proved that the change of bacterial growth environment temperature has a negative impact on bacterial activity,and the in-situ culture system can improve the experimental efficiency and the reliability of experimental results.

关 键 词：原位培养 分布式温度控制 增量式PID 金黄色葡萄球菌 

分 类 号：R331[医药卫生—人体生理学]