IDH突变调控BMP2、COL27A1和SERPINA5基因表达影响人脑胶质瘤的生存预后  

IDH mutation regulates expressions of BMP2,COL27A1,and SERPINA5 to improve survival prognoses of glioma patients

作　　者：包逸雯 朱伟良[1] BAO Yi-wen;ZHU Wei-liang(Department of Oncology,Zhujiang Hospital,Southern Medical University,Guangzhou 510220,China)

机构地区：[1]南方医科大学珠江医院肿瘤科,广州510220

出　　处：《中国临床神经外科杂志》2023年第3期179-183,共5页Chinese Journal of Clinical Neurosurgery

基　　金：广东省自然科学基金(2018A030313846;2021A1515012593)。

摘　　要：目的探讨异柠檬酸脱氢酶(IDH)突变影响胶质瘤临床预后的潜在分子机制。方法计算机检索CGGA和TCGA数据库,获取胶质瘤RNA测序数据(RNA-seq),应用生物信息学分析方法分析IDH突变改善胶质瘤临床预后的潜在分子机制。结果从TCGA数据库下载胶质母细胞瘤(GBM)和低级别胶质瘤(LGG)相关RNA-seq,从CGCA数据量下载648例胶质瘤的RNAseq(234例GBM和414例LGG),从GEO数据量下载单细胞RNA-seq数据GSE131928数据集(28例IDH野生型GBM)。GO和KEGG分析显示,IDH突变显著下调胞外基质(ECM)相关基因表达,GEPIA分析显示BMP2、COL27A1、SERPINA5、VEGFA基因表达水平与胶质瘤生存预后有关,多因素Cox比例回归风险模型分析显示BMP2、COL27A1、SERPINA5基因表达是胶质瘤生存预后独立影响因素,BMP2联合SERPINA5预测胶质瘤生存预后效果最好。结论我们结果提示胶质瘤IDH基因突变,可能通过调控BMP2、COL27A1和SERPINA5基因表达,影响病人生存预后。Objective To explore the potential molecular mechanisms of isocitrate dehydrogenase(IDH)mutation improving the clinical prognoses of glioma patients.Methods Glioma RNA sequencing data(RNA-seq)were obtained from CGGA and TCGA databases.The potential molecular mechanisms of IDH mutation to improve the clinical prognoses of glioma patients was analyzed using bioinformatics methods.Results RNA-seq related to glioblastoma(GBM)and low-grade glioma(LGG)were downloaded from the TCGA database,and RNA-seq of 648 gliomas(234 GBM and 414 LGG)were downloaded from CGCA data.Single cell RNA-seq dataset GSE131928(28 IDH wild-type GBM)was downloaded from GEO database.GO and KEGG analyses showed that IDH mutation significantly down-regulated the expression of extracellular matrix related genes.GEPIA analysis showed that the expression levels of BMP2,COL27A1,SERPINA5 and VEGFA genes were correlated with the survival prognoses of glioma patients.Multivariate Cox proportional regression risk model analysis showed that expressions of BMP2,COL27A1,and SERPINA5 genes were independent risk factors for survival prognoses of glioma patients,and BMP2 combined with SERPINA5 was the best predictor for survival prognoses of glioma patients.Conclusions Our results suggest that IDH gene mutation may improve survival prognoses of glioma patients by regulating the expressions of BMP2,COL27A1,and SERPINA5.

关 键 词：胶质瘤 IDH突变 生存预后 基因表达 

分 类 号：R739.41[医药卫生—肿瘤] Q786[医药卫生—临床医学]