ITGA4基因在脑胶质瘤中的表达及临床意义  

Expression of integrinα4 gene in human brain gliomas and its clinical significance based on bioinformatics analysis

作　　者：鲁飞 柳保丽 张万宏 吴恒浩 张圣旭 LU Fei;LIU Bao-li;ZHANG Wan-hong;WU Heng-hao;ZHANG Sheng-xu(Xinxiang Medical University,Xinxiang 453003,China;Department of Neurosurgery,Kaifeng Central Hospital,Kaifeng 475000,China;Department of Neuroelectrobiology,The Second Affiliated Hospital of Xinxiang Medical University,Xinxiang 453002,China)

机构地区：[1]新乡医学院,河南新乡453003 [2]开封市中心医院神经外科,河南475000 [3]新乡医学院第二附属医院神经电生理科,河南新乡453002

出　　处：《中国临床神经外科杂志》2023年第3期184-187,215,共5页Chinese Journal of Clinical Neurosurgery

摘　　要：目的 探讨整合素α4(ITGA4)基因在脑胶质瘤中的表达与临床意义。方法 计算机检索CGGA数据库获取mRNAseq-325数据集中325例脑胶质瘤的转录组测序(RNA-seq)数据及临床资料,并用m RNAseq-693数据集中693例脑胶质瘤进行验证。结果 胶质瘤ITGA4基因呈高表达(P<0.05),与胶质瘤IDH基因型、1p/19q共缺失状态、WHO病理分级有关(P<0.05),与胶质瘤MGMT启动子甲基化状态无明显关系(P>0.05)。多因素Cox回归分析显示,ITGA4高表达是胶质瘤生存预后不良的独立危险因素(P<0.05)。生存曲线分析显示,ITGA4高表达胶质瘤病人生存期明显缩短(P<0.05)。GO分析和KEGG分析显示,ITGA4基因调控的生物过程包括细胞粘附、血管生成及细胞迁移等,主要调控PI3K/AKT信号通路。结论 我们的结果提示胶质瘤ITGA4呈高表达,与胶质瘤不良生存预后密切相关,主要调控PI3K/AKT信号通路。Objective To investigate the expression of integrinα4(ITGA4)gene in human brain gliomas and its clinical significance.Methods The transcriptional sequencing(RNA-seq)data and clinical data of 325 patients with gliomas in mRNAseq-325 dataset were downloaded from CGGA database,and 693 patients with gliomas in mRNAseq-693 dataset were used to verified.Results ITGA4 gene was highly expressed in human brain gliomas(P<0.05),which was related to IDH genotype,1p/19q codeletion status,and WHO pathological grade(P<0.05),and was not relatted to MGMT promoter methylation status(P>0.05).Multivariate Cox regression analysis showed that high expression of ITGA4 was an independent risk factor for poor survival prognosis of glioma patients(P<0.05),and survival curve analysis showed that the survival time of glioma patients with high ITGA4 expression was significantly shortened(P<0.05).GO and KEGG analyses showed that ITGA4 gene were involved in biological processes including cell adhesion,angiogenesis,and cell migration,and mainly involved in the PI3K/AKT signaling pathway.Conclusions Our results suggest that ITGA4 is highly expressed in gliomas,which is closely related to the poor survival prognoses of glioma patients.ITGA4 mainly regulates the PI3K/AKT signaling pathway.

关 键 词：胶质瘤 整合素α4(ITGA4) 基因表达 生信分析 

分 类 号：R739.41[医药卫生—肿瘤] Q786[医药卫生—临床医学]