NH_(2)MIL与半胱氨酸的相互作用及其荧光传感应用  

Interaction of NH_(2)MIL with cysteine and its application in fluorescence sensing

作　　者：闫莹飞 宫雨晓 单丹[1] YAN Yingfei;GONG Yuxiao;SHAN Dan(School of Environmental and Biological Engineering,Nanjing University of Science and Technology,Nanjing 210094,China)

机构地区：[1]南京理工大学环境与生物工程学院,南京210094

出　　处：《扬州大学学报（自然科学版）》2023年第2期12-18,共7页Journal of Yangzhou University：Natural Science Edition

基　　金：国家自然科学基金资助项目(2274075)。

摘　　要：为研究L-半胱氨酸(L-Cys)的荧光检测方法,先将Fe^(3+)和2-氨基对苯二甲酸(NH_(2)BDC)通过水热法聚合制备结晶纳米配位聚合物NH_(2)MIL,再利用NH_(2)MIL中的Fe^(3+)和L-Cys中巯基之间的氧化还原反应使NH_(2)MIL解聚,释放配体NH_(2)BDC,通过对NH_(2)BDC的荧光检测实现L-Cys检测.实验结果表明,悬浮液中L-Cys的浓度和NH_(2)MIL悬浮液的荧光发射强度在10~200 nmol·L^(-1)浓度范围内呈良好的线性关系,R^(2)=0.999 1,检测限达1.78 nmol·L^(-1),且该检测方法对L-Cys有较强的选择性和抗干扰能力,具有一定的使用前景.In order to study the fluorescence detection of cysteine(L-Cys),the crystalline nano-ligand polymer NH_(2)MIL is prepared by hydrothermal polymerization of Fe^(3+)and 2-aminoterephthalic acid(NH_(2)BDC),and then the NH_(2)MIL is depolymerized by the redox reaction between Fe^(3+)in NH_(2)MIL and sulfhydryl group in cysteine.The ligand NH_(2)BDC is released,and L-Cys is detected by fluorescence detection of NH_(2)BDC.The experimental results show that the concentration of L-Cys in the suspension and the fluorescence emission intensity of NH_(2)MIL suspension have a good linear relationship in the concentration range of 10~200 nmol·L^(-1)(R^(2)=0.9991),and the detection limit reaches to 1.78 nmol·L^(-1).Moreover,the detection method has strong selectivity and anti-interference ability for L-Cys,and it has certain application prospect.

关 键 词：半胱氨酸 NH_(2)MIL 解聚 荧光传感 

分 类 号：O657.3[理学—分析化学]