Transcriptional regulation of secondary metabolism and autophagy genes in response to DNA replication stress in Setosphaeria turcica  

作　　者：MENG Ya-nan ZHANG Xin-jie ZENG Fan-li ZHAI Wei-bo LI Pan HU Jing-jing XIAO Sheng-lin HAO Zhi-min CAO Zhi-yan CHEN Chuan DONG Jin-gao 

机构地区：[1]State Key Laboratory of North China Crop Improvement and Regulation,Hebei Agricultural University,Baoding 071001,P.R.China [2]College of Life Sciences,Hebei Agricultural University,Baoding 071001,P.R.China [3]Key Laboratory of Microbial Diversity Research and Application of Hebei Province,School of Life Sciences,Hebei University,Baoding 071001,P.R.China [4]Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology,Hebei Agricultural University,Baoding 071001,P.R.China [5]College of Plant Protection,Hebei Agricultural University,Baoding 071001,P.R.China

出　　处：《Journal of Integrative Agriculture》2023年第4期1068-1081,共14页农业科学学报（英文版）

基　　金：supported by the grants from the Youth Top Talent Project from Hebei Provincial Department of Education,China(BJ2020003);the China Agriculture Research System of MOF and MARA(CARS-02-25);the State Key Laboratory of North China Crop Improvement and Regulation;Open Project of Key Laboratory of Microbial Diversity Research and Application of Hebei Province(MDRA202101);the Hebei Provincial Department of Bureau of Science and Technology(360-0803-JSN-3YGS);the Natural Science Foundation of Hebei Province(C202204138)。

摘　　要：The fungal pathogen Setosphaeria turcica causes northern corn leaf blight(NCLB),which leads to considerable crop losses.Setosphaeria turcica elaborates a specialized infection structures called appressorium for maize infection.Previously,we demonstrated that the S.turcica triggers an S-phase checkpoint and ATR(Ataxia Telangiectasia and Rad3 related)-dependent self-protective response to DNA genotoxic insults during maize infection.However,how the regulatory mechanism works was still largely unknown.Here,we report a genome wide transcriptional profile analysis during appressorium formation in the present of DNA replication stress.We performed RNA-Seq analysis to identify S.tuicica genes responsive to DNA replication stress.In the current work,we found that appressorium-mediated maize infection by S.turcica is significantly blocked by S-phase checkpoint.A large serial of secondary metabolite and melanin biosynthesis genes were blocked in appressorium formation of S.turcica during the replication stress.The secondary metabolite biosynthesis genes including alcohol dehydrogenase GroES-like domain,multicopper oxidase,ABCtransporter families,cytochrome P450 and FAD-containing monooxygenase were related to plant pathogen infection.In addition,we demonstrated that autophagy in S.turcica is up-regulated by ATR as a defense response to stress.We identified StATG3,StATG4,StATG5,StATG7 and StATG16 genes for autophagy were induced by ATR-mediated S-phase checkpoint.We therefore propose that in response to genotoxic stress,S.turcica utilizes ATR-dependent pathway to turn off transcription of genes governing appressorium-mediated infection,and meanwhile inducing transcription of autophagy genes likely as a mechanism of self-protection,aside from the more conservative responses in eukaryotes.

关 键 词：Setosphaeria turcica APPRESSORIUM secondary metabolite AUTOPHAGY DNA replication stress 

分 类 号：S435.13[农业科学—农业昆虫与害虫防治]