高效液相色谱法同时测定菌液中5种有机酸的含量  

Determination of five kinds of organic acids in bacterial liquid by high performance liquid chromatography

作　　者：万欣 江鹏 何苗[1,2] 肖章 王佳 李立欢[1] 刘明学 WAN Xin;JIANG Peng;HE Miao;XIAO Zhang;WANG Jia;LI Li-huan;LIU Ming-xue(School of Life Science and Engineering,Southwest University of Science and Technology,Mianyang 621010,China;Mianyang Product Quality Supervision and Inspection Institute,Mianyang 621010,China;Key Laboratory for Solid Waste Treatment and Resource Recycle of the Ministry of Education,Mianyang 621010,China)

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621010 [2]绵阳市产品质量监督检验所,四川绵阳621010 [3]固体废物处理与资源化教育部重点实验室,四川绵阳621010

出　　处：《现代化工》2023年第4期227-230,236,共5页Modern Chemical Industry

基　　金：国家自然科学基金面上项目(42172338);四川省科技厅项目(2021YJ0327)。

摘　　要：建立了利用高效液相色谱同时测定粪产碱杆菌菌液中5种有机酸代谢产物的分析方法。采用Agilent Eclipse XDB-C18色谱柱(4.6×150 mm,5μm),流动相为V(磷酸氢二钠)∶V(甲醇)=97∶3,流速0.6 mL/min,检测波长210 nm,柱温35℃,进样量20μL。结果表明,该方法在12 min内简便快速地分离出5种有机酸(丁二酸、乳酸、柠檬酸、乙醛酸和苹果酸),且峰形良好、分离度好,线性范围内各酸的浓度与色谱峰面积的线性关系良好,相关系数不低于0.9995,加标回收率为99.22%~103.60%,相对标准偏差小于5%。该方法简便快速,具有前处理简单、分离效果好、精密度高、回收率高等特点,可用于同时测定菌液中5种有机酸的含量。An analytical method for simultaneous determination of five kinds of organic acid metabolites in Alcaligenes faecalis is established by means of high performance liquid chromatography.An Agilent Eclipse XDB-C18 column(4.6×150 mm,5μm)is used,the mixed mobile phase is composed of disodium hydrogen phosphate:methanol=97:3,the flow rate is 0.6 mL·min-1,the detection wavelength is 210 nm,the column temperature is 35℃,and injection volume is 20μL.Results show that this method can easily and quickly separate five kinds of organic acids(succinic acid,lactic acid,citric acid,glyoxylic acid and malic acid)within 10 minutes,shows good peak shape and good resolution,and exhibits good linear relationship between the concentrations of each acid and chromatographic peak area.It delivers a correlation coefficient not less than 0.9995,a standard addition recovery rate of 99.22%-103.60%,and a relative standard deviation less than 5%.It is verified that this method is simple and rapid,and shows the characteristics such as simple pretreatment,good separation effect,high precision and high recovery rate.It can be used to simultaneously determine the contents of five kinds of organic acids.

关 键 词：高效液相色谱 菌液 有机酸 含量测定 

分 类 号：O657.7[理学—分析化学]