基于代谢组学探讨茵陈蒿汤加减方对支气管哮喘湿热证型大鼠的调控机制  

Study on the regulatory mechanism of modified Yinchenhao Decoction on rats with bronchial asthma damp-heat syndrome based on metabolomics

作　　者：贺一玮 桑勉[1] 孙洮玉[1] 徐荣谦[1] HE Yiwei;SANG Mian;SUN Taoyu;XU Rongqian(Department of Pediatrics,Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing100700,China)

机构地区：[1]北京中医药大学东直门医院儿科,北京100700

出　　处：《中国医药导报》2023年第12期9-13,共5页China Medical Herald

基　　金：国家自然科学基金青年科学基金资助项目(81704120);北京中医药大学青年教师项目(2019-JYB-JS-068)。

摘　　要：目的从代谢组学角度探究茵陈蒿汤加减方对支气管哮喘(以下简称“哮喘”)湿热证型大鼠的调控机制。方法将65只SPF级雄性SD大鼠,4周龄,(180±10)g,分为空白组(10只)、模型组(10只)、西药组(15只)、中药组(15只),使用卵清蛋白氢氧化铝生理盐水混悬液腹腔注射致敏,卵清蛋白雾化激发哮喘,高脂喂养、大肠埃希菌灌胃、置于湿热环境等方式建立湿热证模型,造模成功后,中药组予茵陈蒿汤加减方,西药组予地塞米松,空白组和模型组予生理盐水,灌胃4周。观察大鼠一般状态,筛选差异代谢物,进行代谢途径分析。结果与模型组比较,用药后,中药组精神、饮食、二便状态改善,反应灵敏,呼吸逐渐平稳,口鼻分泌物减少;中药组11,14,17-二十碳三烯酸、二十二碳六烯酸、二十二碳五烯酸、α-亚麻酸、亚油酸等各类脂肪酸及L-色氨酸、L-赖氨酸水平上调,L-组氨酸水平下调。代谢通路中与亚油酸代谢相关性最强,其次为α-亚麻酸代谢、花生四烯酸代谢、色氨酸代谢、组氨酸代谢、精氨酸和脯氨酸代谢。结论茵陈蒿汤加减方对湿热型哮喘有治疗作用,机制可能与脂肪酸、氨基酸代谢调节有关。Objective To investigate the regulatory mechanism of modified Yinchenhao Decoction on rats with bronchial asthma(“asthma”for short)damp-heat syndrome based on metabolomics.Methods A total of 65 SPF male SD rats,4 weeks old,(180±10)g,were divided into blank group(10 rats),model group(10 rats),western medicine group(15 rats),and traditional Chinese medicine group(15 rats).The damp-heat syndrome model was established by intraperitoneal injection of Ovalbumin Hydroxide saline suspension for sensitization,Ovalbumin to stimulate asthma,high-fat feeding,Escherichia coli gavaged,and placing in a damp-heat environment.After successfully modeling,the traditional Chinese medicine group was given modified Yinchenhao Decoction,the western medicine group was given Dexamethasone,the blank group and the model group were given normal saline for four weeks.The general state of rats was observed,different metabolites were screened,and metabolic pathways were analyzed.Results Compared with model group,after medication,the mental,dietary,and bowel status of traditional Chinese medicine group was improved,the response was sensitive,breathing was gradually stable,and oral and nasal secretions were reduced;the levels of 11,14,17-eicosanotrienoic acid,docosahexaenoic acid,docosapentaenoic acid,α-linolenic acid,L-linoleic acid and L-tryptophan,and L-lysine were increased in the traditional Chinese medicine group,while the levels of histidine were decreased.In the metabolic pathway,it has the strongest correlation with linoleic acid metabolism,followed byα-linolenic acid metabolism,arachidonic acid metabolism,tryptophan metabolism,histidine metabolism,arginine,and proline metabolism.Conclusion Modified Yinchenhao Decoction has therapeutic effect on damp-heat syndrome,and the mechanism may be related to the regulation of fatty acid and amino acid metabolism.

关 键 词：支气管哮喘 茵陈蒿汤加减方 代谢组学 脂肪酸 氨基酸 

分 类 号：R562.25[医药卫生—呼吸系统]