单细胞测序技术在肝脏疾病中的研究进展  

Research progress of single-cell sequencing technology in liver diseases

作　　者：陈思思 任锋[1] 刘梅[1] Chen Sisi;Ren Feng;Liu Mei(Oncology Department of Beijing Youan Hospital Affiliated to Capital Medical University,Beijing Institute of Hepatology,Beijing 100069,China)

机构地区：[1]首都医科大学附属北京佑安医院肿瘤内科,北京肝病研究所,北京100069

出　　处：《中华肝脏病杂志》2023年第2期198-201,共4页Chinese Journal of Hepatology

基　　金：国家自然科学基金项目(81770611);北京自然科学基金和北京市教委联合资助重点项目(KZ202010025035);北京市科技计划"首都临床诊疗技术研究及示范应用"专项课题(Z191100006619096、Z191100006619097);北京市医院管理中心"登峰人才"专项资助项目(DF20221503);首都卫生发展科研专项重点攻关项目(首发2020-1-1151)。

摘　　要：肝脏疾病是全球沉重的疾病负担之一,但鉴于其发病机制十分复杂,治疗选择相对受限,所以需要新技术对其发病机制进行深入研究。单细胞测序(SCS)作为一种新兴测序方法,通过对单个细胞的基因组、转录组和表观基因组测序,反映细胞间的异质性,进而揭示疾病发生和发展过程中的复杂机制。SCS在肝病研究中的应用将丰富我们对肝病发病机制的理解,为探索肝病的诊疗方法提供新方向。现主要对SCS技术在肝脏疾病中的研究进展予以综述。Liver disease is one of the most burdensome diseases in the world.Therefore,new technologies are needed to study its pathogenesis in depth;however,because of its complex pathogenesis,there are relatively limited treatment options.Single-cell sequencing(SCS),as an emerging sequencing method,reflects the heterogeneity between cells by sequencing the genome,transcriptome,and epigenome of a single cell,thereby revealing the complex mechanisms of disease occurrence and development.The application of SCS in the study of liver diseases will enrich our understanding of the pathogenesis of liver diseases and provide a new direction for exploring the diagnosis and treatment.This article mainly reviews the research progress of SCS technology in liver diseases.

关 键 词：肝疾病 单细胞测序 异质性 发病机制 

分 类 号：R575[医药卫生—消化系统]