白及多糖水凝胶的制备、表征及其促染菌伤口愈合作用的研究  

Study on preparation and characterization of Bletilla striata polysaccharide hydrogel and its pro-infected wound healing effect

作　　者：岳璇 张晨 瞿燕[1] YUE Xuan;ZHANG Chen;QU Yan(School of Pharmacy,Chengdu University of Traditional Chinese Medicine,State Key Laboratory of Characteristic Chinese Medicine Resources in Southwest China,Chengdu 611137,Sichuan)

机构地区：[1]成都中医药大学药学院,西南特色中药资源国家重点实验室,四川成都611137

出　　处：《中药与临床》2023年第1期29-35,46,共8页Pharmacy and Clinics of Chinese Materia Medica

基　　金：四川省卫生健康委员会普及应用项目(21PJ108);中国博士后科学基金(No.2021M690488);“杏林学者”学科人才科研提升计划(BSH2020020)

摘　　要：目的:制备没食子酸(gallic acid,GA)修饰的白及多糖(Bletilla striata polysaccharide,BSP)水凝胶,并对其进行表征和生物活性评价。方法:通过化学改性将GA接枝到BSP的糖链上制备GA-BSP聚合物,采用Folin-Ciocalteu比色法测定其接枝率,运用核磁共振氢谱和红外光谱对其进行表征;该聚合物通过与Fe3+进行物理交联形成水凝胶,倒置实验证明水凝胶的成功形成;对该水凝胶的溶胀、形态、力学、组织粘附以及光热等物理特性进行表征;选择金黄色葡萄球菌和大肠杆菌对水凝胶进行体外光热抗菌实验;最后,通过大鼠背部伴细菌伤口模型,评估水凝胶对细菌感染伤口的促愈合作用。结果:Folin-Ciocalteu比色法测定了GA-BSP聚合物的接枝率为117.60 mg·g^(-1);核磁共振氢谱和红外光谱证明了GA的成功接枝;水凝胶的成功制备通过试管倒置试验得到证实;该水凝胶的物理性能表征显示其具有较好的网络结构以及溶胀、力学、组织黏附和光热性能;此外,生物活性评价证明了该水凝胶还具有优异的光热抗菌和促进染菌伤口愈合的能力。结论:通过化学接枝和物理交联形成的GA-BSP水凝胶具有较好的微观形态、溶胀性能、力学性能、组织粘附性能和光热性能。此外,良好的光热抗菌作用进一步加强了该水凝胶促染菌伤口愈合的能力。Objective:To prepare and characterize gallic acid(GA)-modified Bletilla striata polysaccharide(BSP)hydrogel,and evaluate their biological activity.Method:GA-BSP polymer was prepared by grafting GA onto the sugar chain of BSP through chemical modification,and the grafting rate was determined by Folin-Ciocalteu colorimetry,which was characterized using 1H-NMR and FT-IR.The polymer was physically cross-linked with Fe3+to form hydrogel,and inversion experiments were used to demonstrate the successful formation of the hydrogel.The physical properties of the hydrogel were characterized,including swelling,morphology,mechanical,tissue adhesion and photothermal properties.Staphylococcus aureus(S.aureus)and Escherichia coli(E.coli)were selected to perform in vitro photothermal antibacterial experiments on the hydrogel.Finally,the pro-healing effect of the hydrogel on bacterially infected wounds was evaluated by a model of bacterial wound on the back of rats.Result:The grafting rate of GA-BSP polymer determined by Folin-Ciocalteu colorimetry was 117.60 mg·g-1.1H-NMR and FT-IR proved the successful grafting of GA.The successful preparation of the hydrogel was confirmed by test tube inversion experiments.The physical property characterization of the hydrogel showed a good network structure as well as swelling,mechanical,tissue adhesion and photothermal properties.In addition,the bioactivity evaluation demonstrated that the hydrogel also had excellent photothermal antibacterial and pro-infected wound healing abilities.Conclusion:The GA-BSP hydrogel formed by chemical grafting and physical cross-linking has good microscopic morphology as well as swelling,mechanical,tissue adhesion and photothermal properties.In addition,the good photothermal antibacterial effect further enhances the ability of this hydrogel to promote bacterial wound healing.

关 键 词：白及多糖 没食子酸 水凝胶 抗菌 伤口愈合 

分 类 号：R283[医药卫生—中药学]