Effects of docosahexaenoic acid or arachidonic acid supplementation on the behavior of cardiomyocytes derived from human pluripotent stem cells  

作　　者：MIZUNA YANO KOTA HIROI TETSUYA YUASA KENJI INOUE OSAMU YAMAMOTO TAKAO NAKAMURA DAISUKE SATO ZHONGGANG FENG 

机构地区：[1]Graduate School of Science and Engineering,Yamagata University,Yonezawa,9928510,Japan [2]Faculty of Health Sciences,Japan Healthcare University,Sapporo,0620053,Japan

出　　处：《BIOCELL》2023年第5期1095-1106,共12页生物细胞（英文）

基　　金：supported financially in part by Grants-in-Aid for Scientific Research(C)(21K12661)from the Japan Society for the Promotion of Science and Grant 12-003-111 from Takahashi Industrial and Economic Research Foundation.

摘　　要：Background:Human heart changes its energetic substrates from lactate and glucose to fatty acids during the neonatal period.Noticing the lack of fatty acids in media for the culture of cardiomyocytes derived from human pluripotent stem cells(hiPS-CM),researchers have supplemented mixtures of fatty acids to hiPS-CM and reported the enhancement in the maturation of hiPS-CM.In our previous studies,we separately supplemented two polyunsaturated fatty acids(PUFAs),docosahexaenoic acid(DHA)or arachidonic acid(AA),to rat fetal cardiomyocytes and found that the supplementations upregulated the expressions of mRNAs for cardiomyocyte differentiation,fatty acid metabolism,and cellular adhesion.The enhancement in cellular contractility was attributed to the improvement in intercellular connection rather than a direct enhancement of the contractile force.Methods:This study reports the successive results of the effects of DHA or AA supplementation on hiPS-CM.In addition to the contractile force and mRNA measurements used in the previous study,we further investigated the effect of different cellular aggregations on the contractile force output by means of finite element analysis,measured glucose and fatty acids metabolites,and assessed cTNT and MLC2v expressions through immunofluorecsence evaluation.Results:It showed that the sole supplementation of albumin-conjugated DHA or AA can be taken up by hiPS-CM without other uptake-enhancing factors,and the supplementations may activate the CD36_ERRγmetabolic pathway.DHA or AA supplementation increased the cellular contractile ratio on collagen gels and AA supplementation stimulated hiPS-CM aggregation to form cellular clusters.The enhancement effect on the hiPS-CM contractile force was modest since the increase in contractile force was not significant.AA supplementation was more effective than DHA supplementation because it significantly upregulated mRNA expressions of P300 and CD36.However,finite element analysis showed that the formation of clusters on a collagen gel attenuated the

关 键 词：Human iPS cells CARDIOMYOCYTES Polyunsaturated fatty acid mRNA expression CONTRACTILITY Fatty acid metabolism 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]