十溴二苯乙烷(DBDPE)在斑马鱼体内的生物富集和生物转化  

Bioaccumulation and biotransformation of decabromodiphenyl ethane(DBDPE)in zebrafish

作　　者：古顺茜 唐斌 蔡凤珊 严骁 姚丽君 李嘉璐 姚丽丽 谢春[1] 郑晶 GU Shunxi;TANG Bin;CAI Fengshan;YAN Xiao;YAO Lijun;LI Jialu;YAO Lili;XIE Chun;ZHENG Jing(School of Public Health,the key Laboratory of Environmental Pollution Monitoring and Disease Control,Ministry of Education,Guizhou Medical University,Guiyang,550025,China;State Environmental Protection Key Laboratory of Environmental Pollution Health Risk Assessment,Research Group of Emerging Contaminants,South China Institute of Environmental Sciences,Ministry of Ecology and Environment,Guangzhou,510655,China;State Key Laboratory of Organic Geochemistry,Guangzhou Institute of Geochemistry,Chinese Academy of Sciences,Guangzhou,510640,China;University of Chinese Academy of Sciences,Beijing,100049,China)

机构地区：[1]贵州医科大学公共卫生与健康学院,环境污染与疾病监控教育部重点实验室,贵阳550025 [2]生态环境部华南环境科学研究所,新污染物研究团队,国家环境保护环境污染健康风险评价重点实验室,广州510655 [3]中国科学院广州地球化学研究所,有机地球化学国家重点实验室,广州510640 [4]中国科学院大学,北京100049

出　　处：《环境化学》2023年第4期1085-1096,共12页Environmental Chemistry

基　　金：国家自然科学基金-青年科学基金(42007341)资助.

摘　　要：本研究通过染毒鱼食暴露,探讨了十溴二苯乙烷(DBDPE)在斑马鱼不同组织内的生物富集和转化特征.实验包括28 d暴露期和77 d清除期,评估了DBDPE在鱼体各组织的同化效率(α)、清除速率常数(kd)、生物半衰期(t_(1/2))和生物放大因子(BMF)等;并利用高效液相色谱-串联四极杆-飞行时间质谱(UPLC-QTOF-MS)筛查分析血清中DBDPE的代谢产物.研究结果表明,暴露期DBDPE在鱼体各组织中均呈相似的吸收曲线,α介于10.1%±1.34%至18.3%±3.78%之间.鱼体组织中DBDPE的清除遵循伪一级动力学,kd为(3.66±0.16)×10^(−2)·d^(−1)至(4.69±0.06)×10^(−2)·d^(−1),t_(1/2)为(14.8±0.19)d至(19.0±0.83)d.DBDPE在鱼体组织中的BMF介于(0.72±0.04)至(1.12±0.23),具有潜在的生物富集特征,且其生物富集潜力与组织功能及脂肪含量相关.在斑马鱼血清中共鉴定出4种脱溴代谢产物、3种羟基产物和1种甲氧基产物,以及2种甲磺基代谢物;表明DBDPE在斑马鱼体内存在明显的生物转化作用,进而影响其在鱼体内的生物富集潜力.In the present study,bioaccumulation and biotransformation of decabromodiphenyl ethane(DBDPE)in tissues of zebrafish(Danio rerio)were investigated after administered DBDPE to zebrafish via the diet.The experiment consists of a 28-day of uptake period and a 77-day of depuration period.Bioaccumulation constants,including the assimilation efficiency(α),depuration rate constants(kd),half-life(t_(1/2)),and biomagnification factor(BMF),for DBDPE in tissues of zebrafish were calculated.Biotransformation products of DBDPE were screened in serum of zebrafish using ultra-high performance liquid chromatography coupled with quadrupole time-offlight mass spectrometry(UPLC-QTOF-MS),and was confirmed by the MS2 spectrum.Similar absorption curves were found for DBDPE in muscle,liver,brain,and ovary of zebrafish,withαin a range of 10.1%±1.34%to 18.3%±3.78%.Depuration of DBDPE in zebrafish tissues followed a pseudo first-order kinetics,with kd values ranged from(3.66±0.16)×10^(−2)·d^(−1) to(4.69±0.06)×10^(−2)·d^(−1),and t_(1/2) values ranged from(14.8±0.19)d to(19.0±0.83)d,respectively.The BMF of DBDPE in zebrafish tissues were(0.72±0.04)d to(1.12±0.23)d,indicating a potential bioaccumulation capacity of DBDPE in fish,which was also significantly correlated to the functions and fat content of individual tissues.Four debromination,three hydroxylation,one methoxy,and two methylsulfonyl products of DBDPE were identified in serum of zebrafish.The results of this study demonstrated that DBDPE had obvious biotransformation processes in zebrafish,which affected its bioaccumulation potential in fish.

关 键 词：十溴二苯乙烷 斑马鱼 生物富集 生物转化. 

分 类 号：X171.5[环境科学与工程—环境科学]