人胃黏膜端粒功能障碍与慢性萎缩性胃炎的相关性研究  

Study on the correlation between telomere dysfunction of human gastric mucosa and chronic atrophic gastritis

作　　者：赵兵 周斌[2] Zhao Bing;Zhou Bin(Department of Gastroenterology,Beijing Longfu Hospital,Beijing 100010,China;Endoscopic Center,Guang′anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China)

机构地区：[1]北京市隆福医院消化科,北京100010 [2]中国中医科学院广安门医院内镜中心,北京100053

出　　处：《中华消化杂志》2023年第1期47-51,共5页Chinese Journal of Digestion

摘　　要：目的探讨人胃黏膜端粒功能障碍与慢性萎缩性胃炎(CAG)的相关性。方法选择2019年2月12日至2020年7月10日于中国中医科学院广安门医院内镜中心行胃镜检查并经病理诊断为CAG的患者30例(CAG组),同期选择慢性非萎缩性胃炎(CNAG)患者30例(CNAG组)。采用实时荧光定量聚合酶链反应检测所有患者病理标本的胃黏膜相对端粒长度,采用免疫组织化学染色半定量分析检测端粒重复序列结合因子(TRF)1、TRF2、端粒保护蛋白(POT)1的蛋白质表达水平。采用Spearman相关分析检验胃黏膜相对端粒长度与TRF1、TRF2、POT1蛋白质表达水平的相关性。统计学方法采用Mann-Whitney U检验和独立样本t检验。结果CAG组胃黏膜相对端粒长度短于CNAG组[0.67(0.51,1.17)比1.06(0.69,1.37)],差异有统计学意义(U=297.00,P=0.024)。CAG组TRF1、TRF2、POT1的蛋白质表达水平分别高于CNAG组(4.26±2.49比1.86±1.34、10.12±2.76比8.78±2.81、4.22±2.48比2.53±1.62),差异均有统计学意义(t=8.05、3.23、5.39,P<0.001、=0.001、<0.001)。CAG组胃黏膜TRF2、POT1蛋白质表达水平与胃黏膜相对端粒长度均呈负相关(r=-0.477、-0.417,P=0.008、0.022)。结论端粒功能障碍与CAG的发病存在一定关系,端粒结合蛋白表达水平的改变参与了CAG患者端粒缩短和病理过程。Objective To investigate the correlation between telomere dysfunction of human gastric mucosa and chronic atrophic gastritis(CAG).Methods From February 12,2019 to July 10,2020,at Endoscopy Center,Guang′anmen Hospital,China Academy of Chinese Sciences,30 patients received endoscopy and pathological diagnosed with CAG(CAG group)were collected,and 30 patients with chronic non-atrophic gastritis(CNAG)were collected at the same time(CNAG group).The relative telomere length was detected by real time fluorescent quantitative polymerase chain reaction.The expression of telomere repeat binding factor(TRF)1,TRF2 and protection of telomere(POT)1 at protein level were detected by immunohistochemical staining and semi-quantitative analysis.Spearman analysis was used to analyze the correlation between the relative telomere length of gastric mucosa and the protein expression levels of TRF1,TRF2 and POT1.Mann-Whitney U test and independent sample t test were used for statistical analysis.Results The relative telomere length of the gastric mucosa in the CAG group was shorter than that in the CNAG group(0.67(0.51 to 1.17)vs.1.06(0.69 to 1.37)),and the difference was statistically significant(U=297.00,P=0.024).The protein expression levels of TRF1,TRF2,and POT1 in the CAG group were all higher than those in the CNAG group,respectively(4.26±2.49 vs.1.86±1.34,10.12±2.76 vs.8.78±2.81,4.22±2.48 vs.2.53±1.62),and the differences were statistically significant(t=8.05,3.23,5.39;P<0.001,=0.001,and<0.001).In the CAG group,the protein expression levels of TRF2 and POT1 in gastric mucosa were negatively correlated with the relative telomere length(r=-0.477 and-0.417,P=0.008 and 0.022).Conclusions The telomere dysfunction is related to the pathogenesis of CAG.The change of telomere binding protein expression level is involved in the shortening of telomere and pathological process of CAG patients.

关 键 词：端粒 端粒重复序列结合因子1 端粒重复序列结合因子2 端粒保护蛋白1 慢性萎缩性胃炎 

分 类 号：R573.32[医药卫生—消化系统]