Spindle-E is essential for gametogenesis in the silkworm, Bombyx mori  

作　　者：Kai Chen Xu Yang Dehong Yang Yongping Huang 

机构地区：[1]Jiangsu Key Laboratory of Sericultural Biology and Biotechnology,School of Biotechnology,Jiangsu University of Science and Technology,Zhenjiang,Jiangsu Province,China [2]Key Laboratory of Insect Developmental and Evolutionary Biology,Center for Excellence in Molecular Plant Sciences,Shanghai Institute of Plant Physiology and Ecology,Chinese Academy of Sciences,Shanghai,China [3]CAS Center for Excellence in Biotic Interactions,University of the Chinese Academy of Sciences,Bejing,China

出　　处：《Insect Science》2023年第2期293-304,共12页昆虫科学（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China(31530072,31830093,31601903,32102611);the Natural Science Foundation of Jiangsu Province(BK20210880).

摘　　要：As a defense mechanism against transposable elements,the PIWI-interacting RNA(piRNA)pathway maintains genomic integrity and ensures proper gametogenesis in gonads.Numerous factors are orchestrated to ensure normal operation of the piRNA pathway.Spindle-E(Spn-E)gene was one of the first genes shown to participate in the piRNA pathway.In this study,we performed functional analysis of Spn-E in the model lepidopteran insect,Bombyx mori.Unlike the germline-specific expression pattern observed in Drosophila and mouse,BmSpn-E was ubiquitously expressed in all tissues tested,and it was highly expressed in gonads.Immunofluorescent staining showed that BmSpn-E was localized in both germ cells and somatic cells in ovary and was expressed in spermatocytes in testis.We used a binary transgenic CRISPR/Cas9 system to construct BmSpn-E mutants.Loss of BmSpn-E expression caused derepression of transposons in gonads.We also found that mutant gonads were much smaller than wild-type gonads and that the number of germ cells was considerably lower in mutant gonads.Quantitative real-time PCR analysis and TUNEL staining revealed that apoptosis was greatly enhanced in mutant gonads.Further,we found that the BmSpn-E mutation impacted gonadal development and gametogenesis at the early larval stage.In summary,our data provided the first evidence that BmSpn-E plays vital roles in gonadal development and gametogenesis in B.mori.

关 键 词：Bombyx mori GAMETOGENESIS piRNA pathway Spindle-E 

分 类 号：Q96[生物学—昆虫学]